PURIFICATION

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Target-directed isolation and identification of a serum lectin from lamprey (Lampetra japonica) by chromatographys and MALDI-TOF/TOF
《Acta Oceanologica Sinica》2018年第5期113-116,共4页HAN Yinglun GOU Meng SONG Xiaoping SONG Tao SHI Biyue PANG Yue LI Qingwei 
The National Program on Key Basic Research Project(973 Program)of China under contract No.2013CB835304;the National Marine Public Projects under contract No.201305016;the National Natural Science Foundation of China under contract Nos31772884 and 31601865;the Key Projects of Scientific Research Platform of Liaoning Provincial Education Department under contract No.L201683651
A 105-k Da polymer lectin was purified from lamprey（Lampetra japonica） serum by chromatography methods including cation ion-exchange chromatography with a SP-Sepharose TM XL column and size exclusion chromatography ...
关键词：Lampetra japonica lectin purification identification MALDI-TOF/TOF 
Purification of a Diatom and Its Identification to Cylindrotheca closterium被引量：1
《Journal of Ocean University of China》2015年第2期357-361,共5页WANG Song ZHANG Lin YANG Guanpin ZHU Baohua PAN Kehou 
supported by the Major State Basic Research Development Program of China (973 Program) (2011CB200901);National Technical Supporting Project Foundation (2011BAD14B01);Energy Project from State Bureau of Oceanic Administration (Grant No. GHME2011SW03)
A diatom was purified with colony selection and continuous dilution methods. It was identified to Cylindrotheca closterium according to its morphological characteristics and rbc L and 18 s r RNA gene sequences. The al...
关键词：Cylindrotheca closterium antibiotics sensitivity fatty acid composition total lipid content 
A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification被引量：2
《Journal of Animal Science and Biotechnology》2015年第1期22-28,共7页Kang Kang Keli Yang Jiasheng Zhong Yongxiang Tian Limin Zhang Jianxin Zhai Li Zhang Changxu Song Christine Yuan Gou Jun Luo Deming Gou 
supported in part by the National Basic Research Program of China(973 Program,2012CB124701);National Natural Science Foundation of China No.81170047,81370151(to DG);Shenzhen overseas high-level talentsinnovation program No.YFZZ20111009(to DG);Shenzhen Nanshan Core Technology Program No.KC2013JSJS0020A;Shenzhen Municipal Basic Research Program No.JCYJ20130329120507746(to KK);Postdoctoral Science Foundation of China No.2013 M542203(to KK);Hubei Province Research and Development Project No.2011BBB080(to KY);Project supported by the Key Natural Science Foundation of Hubei Province,China No.2012FFA067(to YT);the Opening Subject of Hubei Key Laboratory of Animal Embryo and Molecular Breeding No.2012ZD156(to KY)
Background： Porcine reproductive and respiratory syndrome virus （PRRSV）, and particularly its highly pathogenic genotype （HP-PRRSV）, have caused massive economic losses to the global swine industry. Results： To ...
关键词：Highly pathogenic Porcine reproductive and respiratory syndrome virus Real-time RT-PCR 
Brain-derived neurotrophic factor induces neuron-like cellular differentiation of mesenchymal stem cells derived from human umbilical cord blood cells in vitro被引量：8
《Neural Regeneration Research》2011年第13期972-977,共6页Lei Chen Zhongguo Zhang Bing Chen Xiaozhi Liu Zhenlin Liu Hongliang Liu Gang Li Zhiguo Su Junfei Wang Guozhen Hui 
the National Basic Research Program of China(973 Program),No. 2005CB522604
Human umbilical cord blood was collected from full-term deliveries scheduled for cesarean section. Mononuclear cells were isolated, amplified and induced as mesenchymal stem cells. Isolated mesenchymal stem cells test...
关键词：human umbilical cord blood purification and culture brain-derived neurotrophic factor neuron-like cells neural regeneration 
Herpes Simplex Virus Type 1 ICP27 Protein:Its Expression, Purification and Specific Antiserum Production
《Virologica Sinica》2010年第3期199-205,共7页Lei ZHAO Xiao-ming REN Alan C. ZHENG 
The startup fund of the hundred talents program of the Chinese academy of science(20071010- 141);National natural science foundation of China (30870120);Open research fund program of the state key laboratory of virology of China (2007003, 2009007);Hubei province natural science foundation of innovation groups project (2008CDA013);Major state basic research development program (973 Program) of China (2010CB 530105)
Herpes simplex virus type 1 (HSV-1) is the causative agent of cold sores and other more serious diseases. HSV-1 infected-cell protein 27 (ICP27) is an immediate-early regulatory phosphoprotein homologous to gene produ...
关键词：Herpes simplex virus type 1 （HSV-1） Infected-cell protein 27 （ICP27） Recombinant protein ANTISERUM Immunofluorescence assay. 
Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection
《Journal of Pharmaceutical Analysis》2010年第2期97-101,共5页Qin Xiao1,2,Hui Chen2,Jin-Ming Lin2 1. College of Ocean,Hebei Agricultural University,Qinhuangdao 066003 2. The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,Department of Chemistry,Tsinghua University,Beijing 100084,China. 
supported by the National Key Technology R & D Program (2006BAK02A13);the National Basic Research Program of China (973Program, No.2007CB714507)
A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET...
关键词：ATP bioluminescence bacterial detection EXPRESSION firefly luciferase PURIFICATION 
Expression and Purification of the DNA Binding Domain of the Epstein-Barr Virus Nuclear Antigen 1
《生物技术通报》2008年第S1期405-410,共6页Meiru Hu~1 Ru Wei~(1,2)Lu Qian~1 Ming Yu~1 Ming Shi~1 Kun He~3 Jie Wang~3 Beifen Shen~1 Ning Guo~1 (1 Institute of Basic Medical Sciences,Beijing 100850 2 North China Coal Medical College 3 National Center of Biomedical Analysis,Beijing 100071) 
supposed by National Basic Research Program of China(973 Program)(2006CB504305);Beijing Natural Science Foundation(No.7051006).
To overexpress EBNA-1 in E.coli and generate the specific antibody,in this study,the antigenicity,epitope and hydrolysis of EBNA-1 were analyzed using the computer design software Biosun.Based on the prediction by com...
关键词：EBNA-1 EXPRESSION PURIFICATION E.COLI 
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