The rapid epidemic of highly pathogenic A/H5N1 avian influenza virus by transmission from poultry to humans triggered global unrest in the pandemic of novel influenza. If a human trophic strain of avian influenza viru...
We previously developed radioimmunoassays (RIAs) for digitoxin and digoxin using antisera raised against digitoxin 3’-hemisuccinate-bovine serum albumin and digoxin 3’-hemisuccinate-bovine serum albumin conjugates, ...
Supported by National Transgenic Major Program of China(2009ZX08007-006B);the National Natural Science Foundation of China(31072160);Science and Technique Foundation of Shandong Province(2009GG20002032);Natural Science Foundation ofShandong Province(Y2008D20);an Open Issue of State Key Laboratory of Veterinary Biotechnology Fund(SKLVBF200806)~~
[Objective] The aim was to study the prokaryotic expression of P1 gene of foot-and-mouth disease virus(FMDV)type Asia 1and the preparation of its antiserum.[Method]The P1 gene of FMDV type Asia 1 was obtained by gen...
The startup fund of the hundred talents program of the Chinese academy of science(20071010- 141);National natural science foundation of China (30870120);Open research fund program of the state key laboratory of virology of China (2007003, 2009007);Hubei province natural science foundation of innovation groups project (2008CDA013);Major state basic research development program (973 Program) of China (2010CB 530105)
Herpes simplex virus type 1 (HSV-1) is the causative agent of cold sores and other more serious diseases. HSV-1 infected-cell protein 27 (ICP27) is an immediate-early regulatory phosphoprotein homologous to gene produ...
The Key Project of the Ministry of Education of China (108028);National Natural Science Foundation of China (3090068);Grant from State Key Laboratory for Infectious Diseases Prevention and Control, SKL (2008SKLID310)
Viruses (e.g. Human immunodeficiency virus, Human simplex virus and Prototype foamy virus) are obligate intracellular parasites and therefore depend on the cellular machinery for cellular trafficking. Bovine foamy vir...
the Hi-Tech Research and Development Program of China (863 Program, No. 2006AA02A128);Natural Science Foundation of Jiangsu Province, China (No. BK2008010)
Objective To prepare and identify a polyclonal antibody against rat myostatin and investigate myostatin expression in the rat atrophic gastrocnemius muscle after tibial nerve crush. Methods The purified fusion protein...
financially supported by the following funds to Zi-Chun Hua: the National Nature Science Foundation of China (No. 30425009, 30600320, 30730030, 30330530, 30270291);Jiangsu Provincial Nature Sciences Foundation (No. BK2007715);Jiangsu Provincial Department of Health (No. H200524, H200742)
The wild-type human Fas-associated death domain (FADD) protein was expressed as a His-tag fusion protein in Escherichia coll. Recombinant FADD proteins were purified under the denatured condition. After denatured pr...
National Natural Science Foundation ofChina (30570072, 30770097);Natural Science Foun-dation of Tianjin (05YFJM- JC01000).
The Borfl protein is encoded by an immediate-early gene of the bovine foamy virus (BFV) and plays a key role in the viral life cycle. Borfl is a DNA binding protein which can transactivate both the long terminal rep...
A laccase gene from Trametes sp. 420 was recombinantly expressed in Pichia pastoris, producing the enzyme rLacD. Six mutant enzymes were produced by site-directed mutation at six potential glycosylation sites in the e...
The hapten, 3-{ [1-(3-(methyl)phenyloxy)-carbonyl]amino}propanoic acid (HOM), mimicking the analyte metolcarb, was synthesized and verified by mass spectrometry (MS) and 1H-nuclear magnetic resonance spectrome...