SCNT

作品数:38被引量:118H指数:6
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相关领域:农业科学生物学更多>>
相关作者:吴燕妮廖世军张志人刘黎明许卫华更多>>
相关机构:西北农林科技大学吉林大学吉林农业大学甘肃农业大学更多>>
相关期刊:《Science China(Life Sciences)》《新材料产业》《Genes & Diseases》《中国兽医杂志》更多>>
相关基金:国家自然科学基金国家高技术研究发展计划国家重点基础研究发展计划国家教育部博士点基金更多>>
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Highly efficient generation of GGTA1 knockout pigs using a combination of TALEN m RNA and magnetic beads with somatic cell nuclear transfer被引量:7
《Journal of Integrative Agriculture》2016年第7期1540-1549,共10页FENG Chong LI Xi-rui CUI Hui-ting LONG Chuan LIU Xia TIAN Xing-hua PAN Deng-ke LUO Yu-zhu 
supported by the National Basic Research Program of China(973 Program)(2015CB554103 and 2011CBA01004)
The transcription activator-like effector nuclease (TALEN) technique combined with the somatic cel nuclear transfer (SCNT) method has been successfuly applied for creating geneticaly modiifed pigs. However, method...
关键词:transcription activator-like effector nuclease (TALEN) magnetic beads somatic cel nuclear transfer (SCNT) off-target geneticaly modiifed pigs 
Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer被引量:9
《Science China(Life Sciences)》2014年第2期263-268,共6页BAO Lei CHEN HaiDe JONG UiMyong RIM CholHo LI WenLing LIN XiJuan ZHANG Dan LUO Qiong CUI Chun HUANG HeFeng ZHANG Yan XIAO Lei FU ZhiXin 
supported by grants from Ministry of Science and Technology of China(2011CBA01001,2012AA020503);the National Science Fund for Distinguished Young Scholars(31025016);the National Natural Science Foundation of China(31271577);Novel Agricultural Variety Breeding Project of Zhejiang Province(2012C12906-8);the Fundamental Research Funds for the Central Universities;the Key Construction Pro-gram of the National"985"Project(118000+193411801/006);the Research Fund for the Doctoral Program of Higher Education of China(20120101110089)
Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extremely inefficient.Zinc-finger nuclease(ZFN)technology has be...
关键词:PIG XENOTRANSPLANTATION ZFNs GGTA1 biallelic knockout SCNT 
Apoptosis of transgenic cloned and recloned bovine blastocysts被引量:1
《Progress in Natural Science:Materials International》2009年第7期821-826,共6页Guojie Sun Rong Li Yunping Dai Haiping Wang Lili Wang Ying Liu Fangrong Ding Hengxi Wei Ning Li 
supported by the National Basic Research Program of China (Grant No. 2006CB102100);the Natural Science Foundation of Beijing (GrantNo. 5030001)
Apoptosis plays an important role in preimplantation embryonic development. Investigating mechanisms of apoptosis can provide useful information for obtaining high-quality embryos and help to improve cloning efficienc...
关键词:APOPTOSIS Transgenic SCNT blastocysts Transgenic recloned blastocysts BOVINE 
Effect of leptin on oocyte maturation and subsequent pregnancy rate of cloned embryos reconstructed by somatic cell nuclear transfer in pigs被引量:1
《Progress in Natural Science:Materials International》2008年第12期1583-1587,共5页Hengxi Wei Qiuyan Li Jun Li Yan Li Yunping Dai Yufang Ma Kai Xue Ning Li 
supported by the National Basic Research Program of China (Grant No.2007CB947400);Natural Scientific Foundation of Beijing (Grant No.5030001)
Cloning pigs by somatic cell nuclear transfer (SCNT) has wide applications in basic research, human medicine and agricultural production. To improve cloning efficiency, the effect of two basic maturation media, NCSU...
关键词:LEPTIN OOCYTES IVM SCNT PIG 
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