supported by the National Basic Research Program of China(973 Program)(2015CB554103 and 2011CBA01004)
The transcription activator-like effector nuclease (TALEN) technique combined with the somatic cel nuclear transfer (SCNT) method has been successfuly applied for creating geneticaly modiifed pigs. However, method...
supported by grants from Ministry of Science and Technology of China(2011CBA01001,2012AA020503);the National Science Fund for Distinguished Young Scholars(31025016);the National Natural Science Foundation of China(31271577);Novel Agricultural Variety Breeding Project of Zhejiang Province(2012C12906-8);the Fundamental Research Funds for the Central Universities;the Key Construction Pro-gram of the National"985"Project(118000+193411801/006);the Research Fund for the Doctoral Program of Higher Education of China(20120101110089)
Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extremely inefficient.Zinc-finger nuclease(ZFN)technology has be...
supported by the National Basic Research Program of China (Grant No. 2006CB102100);the Natural Science Foundation of Beijing (GrantNo. 5030001)
Apoptosis plays an important role in preimplantation embryonic development. Investigating mechanisms of apoptosis can provide useful information for obtaining high-quality embryos and help to improve cloning efficienc...
supported by the National Basic Research Program of China (Grant No.2007CB947400);Natural Scientific Foundation of Beijing (Grant No.5030001)
Cloning pigs by somatic cell nuclear transfer (SCNT) has wide applications in basic research, human medicine and agricultural production. To improve cloning efficiency, the effect of two basic maturation media, NCSU...