BACILLUS_THURINGIENSIS

作品数:148被引量:496H指数:10
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Bacillus thuringiensis peptidoglycan hydrolase SleB171 involved in daughter cell separation during cell division
《Acta Biochimica et Biophysica Sinica》2016年第4期354-362,共9页Hua Li Penggao Hu Xiuyun Zhao Ziniu Yu Lin Li 
This work was financially supported by the grants from the National Basic Research Program of China (No. 2013CB127504), the National Natural Science Foundation of China (No. 31270158), and Non- Profit Science and Technology Research Funds of Hubei Province of China (No. 2012DBA10001).
Whole-genome analyses have revealed a putative cell wall hydrolase gene (sleB171) that constitutes an operon with two other genes (ypeB and yhcN) of unknown function in Bacillus thuringiensis BMB171. The putative ...
关键词:Bacillus thuringiensis peptidoglycan hydrolase SleB171 cell division 
The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36~I and 605~I被引量:2
《Journal of Integrative Agriculture》2014年第5期1036-1042,共7页ZHOU Zi-shan LIN Hui-yan LI Ying SHU Chang-long SONG Fu-ping ZHANG Jie 
supported by the National Natural Science Foundation of China (31272115);the National 973 Program of China (2009CB118902)
The novel crylAi gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available inf...
关键词:Bacillus thuringiensis CrylAi toxin active fragment TOXICITY Plutella xylostella 
Penetration of a Single Domain of Bacillus thuringiensis Cry1Ie-Domain I to a Lipid Membrane In vitro被引量:1
《Journal of Integrative Agriculture》2014年第5期1043-1050,共8页GUO Shu-yuan LI Jie CHEN Zhen HE Kang-lai 
funded by the National 973 Program of China (2009CB118902);the Natinoal Natural Science Foundation of China (31171911)
Domain I of the activated Crystal protein from Bacillus thuringiensis has a seven a-helix bundle structure, which is responsible for membrane channel formation in its insecticidal mechanism. Crylle is toxic to Asian c...
关键词:Bacillus thuringiensis Cryl Ie domain I of Cry protein monolayer insertion 
Acquisition of Insect-Resistant Transgenic Maize Harboring a Truncated cry1Ah Gene via Agrobacterium-Mediated Transformation被引量:6
《Journal of Integrative Agriculture》2014年第5期937-944,共8页LI Xiu-ying LANG Zhi-hong ZHANG Jie HE Kang-lai ZHU Li HUANG Da-fang 
the financial support of the Genetically Modified Organisms Breeding Major Project, China (2011ZX08003-001);the National Basic Research Program of China (973 Program, 2009CB118902);the National Natural Science Foundation of China (30970231)
A novel insecticidal gene crylAh was cloned from Bacillus thuringiensis isolate BT8 previously for plant genetic engineering improvement. Truncated active CrylAh toxin has a toxicity level similar to that of the full-...
关键词:Bacillus thuringiensis truncated crylAh gene insectresistant maize stable inheritance 
The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana被引量:5
《Journal of Integrative Agriculture》2013年第3期474-482,共9页JIANG Wei-yu GENG Li-li DAI Ping-li LANG Zhi-hong SHU Chang-long LIN Yi ZHOU Ting SONG Fu-ping ZHANG Jie 
supported by the National Basic Research Program of China (2007CB109203 and 2009CB118902)
Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the...
关键词:Bacillus thuringiensis intestinal bacteria horizontal gene transfer transgenic maize Apis cerana BIOSAFETY 
A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua(Lepidoptera:Noctuidae)被引量:10
《Journal of Integrative Agriculture》2012年第4期628-638,共11页LU Qiong ZHANG Yongojun CAO Guang-chun ZHANG Li-li LIANG Ge-mei LU Yan-hui WU Kong-ming GAO Xi-wu GUO Yu-yuan 
supported by the National Basic Research Program of China(2012CB114104);the National Natural Science Foundation of China (31071694 and 31171858)
Cry toxins produced by Bacillus thuringiensis(Bt) are effective biological insecticides against certain insect species.In this study,bioassay results indicated that Cry1B and Cry1C were toxic to Spodoptera exigua.We...
关键词:Bacillus thuringiensis cadherin-like Spodoptera exigua enhancement toxicity 
A pangenomic study of Bacillus thuringiensis被引量:1
《Journal of Genetics and Genomics》2011年第12期567-576,共10页Yongjun Fang Zhaolong Li Jiucheng Liu Changlong Shu Xumin Wang Xiaowei Zhang Xiaoguang Yu Duojun Zhao Guiming Liu Songnian Hu Jie Zhang Ibrahim Al-Mssallem Jun Yu 
supported by a grant from King Abdulaziz City for Science and Technology,Riyadh,Saudi Arabia(No. KACST 428-29);institutional grant from CAS Key Laboratory of Genome Sciences and Information,Beijing Institute of Genomics, Chinese Academy of Sciences;supported by the grants from the National Basic Research Program(973 Program)(No.2010CB126604);the Special Foundation Work Program(No.2009FY 120100);the Ministry of Science and Technology of the People's Republic of China and from the National Science Foundation of China(No. 31071163).
Bacillus thuringiensis(B.thuringiensis) is a soil-dwelling Gram-positive bacterium and its plasmid-encoded toxins(Cry) are commonly used as biological alternatives to pesticides.In a pangenomic study,we sequenced ...
关键词:Bacillus thuringiensis(B.thuringiensis) Pseudo-chromosome Pangenome 
Temporal allocation of metabolic tolerance to transgenic Bt cotton in beet armyworm,Spodoptera exigua(Hübner)被引量:4
《Science China(Life Sciences)》2011年第2期152-158,共7页GUO JianYing WU Gang WAN FangHao 
supported by the National Basic Research Program of China (Grant No. 2006CB102004);the National Natural Science Foundation of China (Grant No. 30800724);New Detecting Technology of Exogenous Gene Protein (Grant Nos. 2008ZX08012-004 and 2009ZX08011-013B)
Digestive and detoxification enzyme activity and nutrient composition were examined in the body of fourth instar beet armyworms,Spodoptera exigua(Hübner),fed on transgenic Bacillus thuringiensis(Bt) and non-Bt cotton...
关键词:Bacillus thuringiensis Spodoptera exigua detoxification enzyme CARBOXYLESTERASE ACETYLCHOLINESTERASE 
In vivo fluorescence observation of parasporal inclusion formation in Bacillus thuringiensis
《Science China(Life Sciences)》2010年第9期1106-1111,共6页YANG Hui RONG Rong SONG FuPing SUN ChangPo WEI Juan ZHANG Jie HUANG DaFang 
supported by the National Basic Research Program of China (Grant No. 2009CB118902);the National High-Tech Research and Development Program of China (Grant No. 2006AA10A212)
A recombinant gene expressing a Cry1Ac-GFP fusion protein with a molecular mass of approximately 160 kD was constructed to investigate the expression of cry1Ac,the localization of its gene product Cry1Ac,and its role ...
关键词:Bacillus thuringiensis Cry1Ac-GFP fusion protein laser confocal microscopy 
Effects of different brush border membrane vesicle isolation protocols on proteomic analysis of Cry1Ac binding proteins from the midgut of Helicoverpa armigera被引量:3
《Insect Science》2008年第6期497-503,共7页Li-Zhen Chen Ge-Mei Liang Brian G. Rector Jie Zhang Kong-Ming Wu Yu-Yuan Guo 
This paper was contributed to the International Symposium on Insect Midgut Biology, April 7-11, 2008, Guangzhou, China.Acknowledgments This research was supported by the National Natural Science Foundation of China (30771424) and National Basic Research Program of China (973-2007CB 109204). We thank Dr. Jun Zhao (University of West Virginia, USA) for reviewing this manuscript.
Brush border membrane vesicles (BBMV) isolated from insect midguts have been widely used to study CrylA binding proteins. Sample preparation is important in two- dimensional electrophoresis (2-DE), so to determine...
关键词:Bacillus thuringiensis binding protein brush border membrane vesicles Cry1Ac Helicoverpa armigera two-dimensional electrophoresis 
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