This work was financially supported by the grants from the National Basic Research Program of China (No. 2013CB127504), the National Natural Science Foundation of China (No. 31270158), and Non- Profit Science and Technology Research Funds of Hubei Province of China (No. 2012DBA10001).
Whole-genome analyses have revealed a putative cell wall hydrolase gene (sleB171) that constitutes an operon with two other genes (ypeB and yhcN) of unknown function in Bacillus thuringiensis BMB171. The putative ...
supported by the National Natural Science Foundation of China (31272115);the National 973 Program of China (2009CB118902)
The novel crylAi gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available inf...
funded by the National 973 Program of China (2009CB118902);the Natinoal Natural Science Foundation of China (31171911)
Domain I of the activated Crystal protein from Bacillus thuringiensis has a seven a-helix bundle structure, which is responsible for membrane channel formation in its insecticidal mechanism. Crylle is toxic to Asian c...
the financial support of the Genetically Modified Organisms Breeding Major Project, China (2011ZX08003-001);the National Basic Research Program of China (973 Program, 2009CB118902);the National Natural Science Foundation of China (30970231)
A novel insecticidal gene crylAh was cloned from Bacillus thuringiensis isolate BT8 previously for plant genetic engineering improvement. Truncated active CrylAh toxin has a toxicity level similar to that of the full-...
supported by the National Basic Research Program of China (2007CB109203 and 2009CB118902)
Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the...
supported by the National Basic Research Program of China(2012CB114104);the National Natural Science Foundation of China (31071694 and 31171858)
Cry toxins produced by Bacillus thuringiensis(Bt) are effective biological insecticides against certain insect species.In this study,bioassay results indicated that Cry1B and Cry1C were toxic to Spodoptera exigua.We...
supported by a grant from King Abdulaziz City for Science and Technology,Riyadh,Saudi Arabia(No. KACST 428-29);institutional grant from CAS Key Laboratory of Genome Sciences and Information,Beijing Institute of Genomics, Chinese Academy of Sciences;supported by the grants from the National Basic Research Program(973 Program)(No.2010CB126604);the Special Foundation Work Program(No.2009FY 120100);the Ministry of Science and Technology of the People's Republic of China and from the National Science Foundation of China(No. 31071163).
Bacillus thuringiensis(B.thuringiensis) is a soil-dwelling Gram-positive bacterium and its plasmid-encoded toxins(Cry) are commonly used as biological alternatives to pesticides.In a pangenomic study,we sequenced ...
supported by the National Basic Research Program of China (Grant No. 2006CB102004);the National Natural Science Foundation of China (Grant No. 30800724);New Detecting Technology of Exogenous Gene Protein (Grant Nos. 2008ZX08012-004 and 2009ZX08011-013B)
Digestive and detoxification enzyme activity and nutrient composition were examined in the body of fourth instar beet armyworms,Spodoptera exigua(Hübner),fed on transgenic Bacillus thuringiensis(Bt) and non-Bt cotton...
supported by the National Basic Research Program of China (Grant No. 2009CB118902);the National High-Tech Research and Development Program of China (Grant No. 2006AA10A212)
A recombinant gene expressing a Cry1Ac-GFP fusion protein with a molecular mass of approximately 160 kD was constructed to investigate the expression of cry1Ac,the localization of its gene product Cry1Ac,and its role ...
This paper was contributed to the International Symposium on Insect Midgut Biology, April 7-11, 2008, Guangzhou, China.Acknowledgments This research was supported by the National Natural Science Foundation of China (30771424) and National Basic Research Program of China (973-2007CB 109204). We thank Dr. Jun Zhao (University of West Virginia, USA) for reviewing this manuscript.
Brush border membrane vesicles (BBMV) isolated from insect midguts have been widely used to study CrylA binding proteins. Sample preparation is important in two- dimensional electrophoresis (2-DE), so to determine...