Dexmedetomidine Promotes Angiogenesis and Vasculogenic Mimicry in Human Hepatocellular Carcinoma through α^(2)-AR/HIF-1α/VEGFA Pathway  

作　　者：FANG Tao LIN Li YE Zhi Jian FANG Lian SHI Shuai YU Ke Da MIAO Hui Hui LI Tian Zuo 

机构地区：[1]Department of Anesthesiology,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China [2]Department of Anesthesiology,The Affiliated Jinhua Hospital of Wenzhou Medical University,Jinhua 321000,Zhejiang,China [3]IVF centre,The Affiliated Jinhua Hospital of Wenzhou Medical University,Jinhua 321000,Zhejiang,China

出　　处：《Biomedical and Environmental Sciences》2022年第10期931-942,共12页生物医学与环境科学（英文版）

基　　金：supported by the Natural Science Foundation of Zhejiang Province[LY19H160002];the Science and Technology Research Program of Jinhua City[2018-3-001b and 2017-3-020]

摘　　要：Objective Dexmedetomidine(DEX),the most specificα^(2)-adrenergic receptor agonist widely used for its sedative and analgesic properties,has been reported to upregulate HIF-1αexpression to protect hypoxic and ischemic tissues.However,it is largely unclear whether DEX can also upregulate Hypoxiainducible factor-1 alpha(HIF-1α)expression and its downstream vascular endothelial growth factor-A(VEGFA)in cancer tissues with oxygen-deficient tumor microenvironment.Methods We used SMMC-7721 cells,MHCC97-H cells,and a mouse model of orthotopic hepatic carcinoma to explore the effect of DEX on angiogenesis and vasculogenic mimicry(VM)and its mechanism.Under normoxic(20%O^(2))and hypoxic(1%O^(2))conditions,DEX was used to intervene cells,and yohimbine was used to rescue them.Results The results showed that DEX promoted angiogenesis and VM in human liver cancer cells within a certain dose range,and the addition of yohimbine inhibited this effect.DEX could activate HIF-1α/VEGFA pathway,which was further verified by silencing HIF-1α.Consistently,in vivo results also showed that DEX can up-regulate HIF-1α/VEGFA expression,and enhance the number of VM channels and microvessel density(MVD).Conclusion We believe that HIF-1α/VEGFA might be an important signaling pathway by which DEX promotes angiogenesis and VM formation in human hepatocellular carcinoma,whereasα^(2)-adrenergic receptor mediation might be the critical mechanisms.

关 键 词：Hepatocellular carcinoma DEXMEDETOMIDINE YOHIMBINE α^(2)-adrenergic receptor HIF-1A VEGFA Vascular mimicry ANGIOGENESIS 

分 类 号：R614[医药卫生—麻醉学] R735.7[医药卫生—外科学]