Cantharidin suppresses HCT116 colorectal carcinoma cell proliferation and migration by changing the cytoskeleton structure  

作　　者：Xin Zhang Tongtong Sui Qixiang Ma Haozhen Shao Xiaowei Hu Honghao Sheng Zhitao Ma Guangbin Luo 

机构地区：[1]School of Life Sciences,BeUing University of Chinese Medicine,BeUing,PR China [2]Department of Genetics and Genome Sciences,Case Western Reserve University,Cleveland,OH 44f06.USA

出　　处：《Journal of Traditional Chinese Medical Sciences》2018年第3期302-309,共8页中医科学杂志（英文）

摘　　要：Background:Cantharidin (CTD),a natural toxin produced from Chinese blister beetles,has extensive anti-tumor activity.The present study investigated the effect of CTD on a human colon cancer cell line to elucidate potential new insights regarding the mechanism(s) through which CTD exerts its anti-tumor effects.Materials and methods:The inhibitory effect of CTD on human colon cancer HCT116 cells was evaluated using the IncuCyte ZOOMTM analyzer.Apoptotic cells were detected by Annexin V-FITC/PI assay and cell cycle was evaluated with flow cytometry following propidium iodide staining.Alterations in F-actin microfilaments were analyzed by FITC-phalloidin staining and morphological changes were evaluated with a laser scanning confocal microscope.Cell migration assay was carried out to investigate the effects of CTD on migration of HCT116 cells in vitro.Results:CTD exhibited a significant growth inhibitory effect on HCT116 cells accompanied by an increase in G2/M phase cells,without a significant effect on apoptosis.CTD-treated cells also exhibited a dramatic collapse in their microfilament network and a significant reduction in cell adhesion.Conclusion:CTD inhibits growth by increasing G2/M phase cells and decreasing S phase cells,revealing that CTD exerts a significant growth inhibitory effect primarily through an inhibition of cell cycle progression (a cytostatic effect).Moreover,a negative effect on cell migration may also constitute a contributing factor to its anti-tumor potential.These findings suggest the potential use for developing CTD as a novel anti-cancer therapy that targets metastasis Giving full play to CTD may inhibit tumor transfer.

关 键 词：CANTHARIDIN ACTIN CYTOSKELETON Cell adhesion Proliferation MIGRATION 

分 类 号：R73[医药卫生—肿瘤]