Effects of an immunosuppressive active component of Periplocae Cortex (Periploca sepium Bge.) on positive selection of thymocytes in vitro  

作　　者：Huifang Deng Hong Yang Dingnan Wang Baihui Zeng Shaohua Shi Han Yu Yunzhou Li Zongying Xu Xianggen Zhong 

机构地区：[1]School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China [2]School of Continuing Education,Beijing University of Chinese Medicine,Beijing 100029,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2018年第2期161-167,共7页中医科学杂志（英文）

基　　金：This study was supported by the National Natural Science Foundation of China(30901909).

摘　　要：Objective:To determine the effect of an immunosuppressive active component (periploside A) isolated from the stem bark of Periplocae Cortex (Periploca sepium Bge.),a Chinese medicinal herb used in the treatment of rheumatoid arthritis for centuries in China,on positive selection of thymocytes in vitro.Methods:Female C57BL/6 mice at 6 weeks of age were housed in specific pathogen-free conditions.Double-positive thymocytes from C57BL/6 mice were induced into positive selection in vitro with or without periploside A treatment.Cell viability and expression of CD69,CD4,and CD8 were analyzed by flow cytometry.Results:Flow cytometric examination of thymocyte populations revealed that the percentage of CD8+ single-positive thymocytes was decreased by periploside A upon differentiation induced by an anti-CD3 antibody.However,the percentage of CD4+ single-positive thymocytes was decreased by periploside A upon differentiation induced by phorbol 12-myristate 13-acetate/ionomycin.Expression of CD69 plays a major role in prohibiting differentiation of thymocytes.Treatment with periploside A decreased CD69 expression in thymocytes.Conclusion:These results demonstrate that periploside A influences positive selection of thymocytes in vitro.

关 键 词：IMMUNOSUPPRESSIVE active COMPONENT POSITIVE selection THYMOCYTES 

分 类 号：R73[医药卫生—肿瘤]