Regulating effect of borosilicate bioglass extract on the osteoblast proliferation activity and osteogenesis signaling pathway function  

作　　者：Xiao-Hui He Wu-Sheng Chen Yi-Fan Zhuang Dao-Qing Wang Xi-Yi Wang Xin-Hong Zhuang 

机构地区：[1]Department of Orthopedics,Puning Hospital of Traditional Chinese Medicine in Guangdong Province,Puning City,Guangdong Province,515300

出　　处：《Journal of Hainan Medical University》2017年第21期13-16,共4页海南医学院学报（英文版）

摘　　要：Objective: To study the regulating effect of borosilicate bioglass extract on the osteoblast proliferation activity and osteogenesis signaling pathway function. Methods: Osteoblasts MG-63 were cultured and divided into borosilicate group and control group that were treated with the culture medium containing borosilicate bioglass extract and the culture medium without extract respectively. After 24 h of treatment, the cell proliferation activity as well as the expression of proliferation activity markers, Wnt signaling pathway molecules and PI3K/AKT signaling pathway molecules was measured. Results: After 24 h of treatment, MTT cell viability of borosilicate group was significantly higher than that of control group, and ALP, OC, OPN, COL-I, Runx2, Wnt1, Wnt3a, β-catenin, LRP5, LRP6, p-PI3K, p-AKT, Bcl-2 and BMP protein expression in cells were significantly higher than those of control group. Conclusion: Borosilicate bioglass extract can enhance the proliferation activity of osteoblasts by activating Wnt pathway and PI3K/AKT pathway.

关 键 词：OSTEOBLAST BOROSILICATE BIOGLASS Proliferation ACTIVITY Signaling pathway 

分 类 号：R73[医药卫生—肿瘤]