罗非鱼蛋白制取多肽酶解条件的优化  

Optimization of hydrolysis conditions for peptides producing from tilapia protein

作　　者：丁利君[1] 黄小梅[1] 何颖基[1] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006

出　　处：《食品与机械》2008年第2期15-18,37,共5页Food and Machinery

基　　金：广东省科技厅计划项目(项目编号:2006B20401012)

摘　　要：目的:以罗非鱼蛋白为原料,用枯草杆菌蛋白酶对其进行酶解制取多肽,研究其最佳酶解工艺;方法:研究酶解温度、pH值、酶解时间、E:S对酶解液中多肽含量的影响,并通过三元二次回归正交试验对酶解工艺进行优化;结果:肉水比为1∶3(W∶V)作为底物,酶(应用酶液)与底物比E∶S=1%,pH7.5,于65℃恒温酶解4h。此条件下,水解度为38.46%,酶解液中有较多的多肽;结论:本试验结果为罗非鱼的开发利用提供了科学的理论依据。Objective:The optimum hydrolysis conditions for peptides producing from tilapia protein were studied.Methods:The protein was hydrolyzed by Alcalase 2.4 L,The influence of temperature,pH,time and E:S of hydrolysis to the content of peptides in the protein hydrolysates were studied.And optimization of the conditions for hydrolysis was ensured by regressive orthogonals experiment.Results:The optimum condition for hydrolysis with alcalase was pH 7.5,the ratio of fish flesh to water 1∶3(W∶V),E∶S=1%(applied alcal...

关 键 词：非洲鲫鱼 碱性蛋白酶 多肽 酶解试验 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]