碱法制备蚕蛹蛋白浸提条件的优化  

Optimization of the alkaline extraction of silkworm(Bombyx mori L.) pupae protein

作　　者：孙雁[1] 任发政[1] 范金波[1] 吴丽萍[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院教育部北京市功能乳品重点实验室,北京100083

出　　处：《农业工程学报》2009年第2期285-289,共5页Transactions of the Chinese Society of Agricultural Engineering

摘　　要：该文通过中心组合设计及相应面分析,研究了碱法制备蚕蛹蛋白的浸提条件对蚕蛹蛋白浸提率的影响,得到了蚕蛹蛋白提取率的数学模型。优化了蚕蛹蛋白的浸提的条件,提高了碱法制备蚕蛹蛋白的蛋白提取率。结果表明NaOH浓度和液固比(V/m)对蚕蛹蛋白提取率影响显著(P<0.05)。蚕蛹蛋白浸提的最佳工艺条件为:NaOH浓度1.03%、液固比(V/m)70︰1、浸提温度50℃、NaCl浓度0.070 mol/L。在此最佳工艺条件下蚕蛹蛋白的实际提取率达78.69%。利用优化工艺条件制备的桑蚕蛹蛋白,其蛋白含量为96.70%,必需氨基酸占总氨基酸含量的47.1%, 必需氨基酸与非必需氨基酸的比值为0.9,具有较好的应用价值。Optimization of the extraction of silkworm(Bombyx mori L.)pupae protein was investigated using response surface methodology and central composite design.The influences of the variables of protein extraction processes were studied and the regression equation of protein extraction rate was established.Protein extraction rate was primarily affected by alkali concentration and liquid/solid ratio(P<0.05), and was remarkably improved to 78.69%under the optimum conditions,when alkali concentration,liquid/solid ...

关 键 词：蚕蛹蛋白 碱提 中心组合设计 响应面 提取率 

分 类 号：TS201.1[轻工技术与工程—食品科学]