气相色谱-氢火焰离子检测器检测海产品中的二甲胺  

Determination of Dimethylamine in Sea Food by Gas Chromatography Coupled with Hydrogen Flame Ionization Detector (GC-FID)

作　　者：贾佳[1] 朱军莉[1] 励建荣[1] 

机构地区：[1]浙江工商大学食品,生物与环境工程学院浙江省食品安全重点实验室,浙江杭州310035

出　　处：《食品科学》2009年第6期167-170,共4页Food Science

基　　金：国家"863"计划重点项目(2007AA091806);浙江省科技厅重大科技攻关项目(2005C12030)

摘　　要：采用气相色谱-FID检测器对海产品中的二甲胺进行检测。本实验对样品中二甲胺的提取、衍生条件以及气相色谱的分析方法进行了研究。样品经7.5%三氯乙酸提取,取上清液在60℃下与对甲苯磺酰氯反应1h,衍生产物在确定的气相色谱条件下进行分析。结果显示:二甲胺在0.001～2mg/ml的浓度范围内线性相关系数为0.9998,日内精密度RSD为2.8%,日间精密度RSD为5.0%。最低检出限为0.38μg/ml,定量限为0.71μg/ml。回收率在91.21%～103.24%之间。该方法操作简单,灵敏度高,稳定性好。An GC-FID method for determination of dimethylamine (DMA) in sea food was developed in this study. The sea food samples were extracted with 7.5% TCA. The supernatant reacted with p-touluenesulfonyl chloride for 1 hour at 60 ℃, and the product of derivatization reaction was analyzed using GC method. The results showed that the correlation coefficient between peak area and DMA concentration in the range of 0.001 mg/ml to 2 mg/ml is 0.9998. The intra-day and inter-day precision RSDs are 2.8% and 5.0%, respecti...

关 键 词：二甲胺 海产品 气相色谱-氢火焰离子检测器 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]