树舌合成碳酸酐酶抑制剂的营养需求  

The Nutritional Requirement to Synthesize Carbonic Anhydrase Inhibitors by Ganoderma applanatum

作　　者：黄达明[1] 刘芹[1] 张志才[1] 张玲[1] 仲媛[1] 赵继友[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《中国食用菌》2009年第6期32-34,共3页Edible Fungi of China

基　　金：国家973基金项目(40721002);江苏安慧生物科技有限公司资助项目(项目编号2006278);江苏大学高级人才专项基金资助(项目编号1283000268)

摘　　要：探索树舌发酵产生高活性的碳酸酐酶抑制剂的营养需求。试验以抑制碳酸酐酶活性为指标,通过单因素试验筛选出适宜碳源、氮源及复合营养因子,通过正交试验进一步观察了各组分之间的相互作用。试验结果表明,树舌发酵合成碳酸酐酶抑制剂的较为适合的培养基为:果糖20 g.L-1、NaNO3 2 g.L-1、马铃薯173 g.L-1、(NH4)2SO4 1.3 g.L-1。使用该培养基其发酵液对碳酸酐酶的抑制率可达91.43%,显著高于未优化的培养基(69.81%)。In this study,the nutritional needs of Ganoderma applanatum to produce high active carbonic anhydrase inhibitors was investigated.Firstly,optimal carbon and nitrogen sources,and natural components were selected by single factor experi-ments according to carbonic anhydrase inhibition ratio of the fermentation broth.Then,inter-action of these factors was observed by orthogonal testsstyle.Finally,the inhibitory ratio based on basal medium and optimized medium was compared.The opti-mized medium contained: fruct...

关 键 词：树舌 碳酸酐酶抑制剂 培养基优化 

分 类 号：R151[医药卫生—营养与食品卫生学]