PITC柱前衍生高效液相色谱法测定黄酒中17种氨基酸方法研究  

Simultaneous Determination of 17 Amino Acids in Chinese Rice Wine by HPLC With Pre-column PITC Derivation

作　　者：钟其顶[1,2] 高红波[1,2] 熊正河[1] 邹慧君[3] 毛严根 谢广发[3] 邢江涛[1,2] 

机构地区：[1]中国食品发酵工业研究院,北京100027 [2]全国食品发酵标准化中心,北京100027 [3]中国绍兴黄酒集团有限公司,浙江绍兴312000 [4]上海金枫酿酒有限公司,上海201501

出　　处：《酿酒》2010年第5期74-76,共3页Liquor Making

摘　　要：建立了柱前衍生高效液相色谱法同时测定黄酒中17种氨基酸的方法。方法采用异硫氰酸苯酯(PITC)为柱前衍生试剂,色谱柱为C18(4.6×250mm,5μm),乙酸钠-乙腈-水混合体系梯度洗脱,紫外检测器,检测波长254nm,40min内17种氨基酸得到良好的分离。在0.025～1.25mmol/L范围内其浓度与响应值具有良好的线性关系,相关系数大于0.99;样品的平均加标回收率在78.6～118.1%之间,相对标准偏差在0.45%～3.30%范围;检出限为0.259～0.925μmol/L,定量限为0.863～3.083μmol/L。相对其他柱前衍生测定氨基酸的方法而言,该方法在可操作、低成本、易推广等方面具有明显优势,可供黄酒中氨基酸测定方法行业标准制定提供参考。A reversed-phase high performance liquid chromatographic(HPLC) method for simultaneous determination of 17 amino acids in Chinese rice wine with pre-column derivation was developed.The sample was derived with phenylisothiocyanate(PITC) and analyzed on C18 column(4.6×250mm,5μm) with detection at 254nm..Using this method,17 amino acids can be separated well in 40min by gradient elution with mix mobile phase of acetonitrile,sodium acetate solution and water.The linear range investigated in this method was 0.02...

关 键 词：黄酒 柱前衍生 异硫氰酸苯酯 氨基酸 标准 

分 类 号：TS262.4[轻工技术与工程—发酵工程]