海洋假单胞菌胞外多糖的抗氧化性  

Antioxidant effects of extracellular polysaccharides from a marine Pseudomonas

作　　者：孙红梅[1] 张星星[1] 白玉[1] 王际辉[1] 王晗[1] 肖珊[1] 叶淑红[1] 

机构地区：[1]大连工业大学辽宁省食品生物技术重点实验室,辽宁大连116034

出　　处：《大连工业大学学报》2012年第6期395-398,共4页Journal of Dalian Polytechnic University

基　　金：国家农业科技成果转化资金项目(2010GB2B000092);国家星火计划项目(2010GA651004);辽宁省高等学校优秀人才支持计划项目(LR2011012);辽宁省高等学校优秀人才支持计划项目(LJQ2011055);辽宁省科学技术基金项目(201202013);大连市科技计划项目(2012B14NC128)

摘　　要：利用海洋假单胞菌PT-8发酵胞外多糖,对其发酵液进行乙醇沉淀,Sevag法除蛋白,透析、冷冻干燥后得到纯化的胞外多糖(EPSs)。用分光光度法测定多糖的还原力以及在不同体系中对羟自由基、DPPH自由基和超氧阴离子自由基的清除能力。结果表明,多糖具有较强的还原力、羟自由基和超氧阴离子的清除能力,多糖对DPPH的清除作用较弱,低于同等条件下的维生素C。A marine pseudomonas PT-8 was fermented to produce extracellular polysaccharides(EPSs).The fermentation liquor was treated in alcohol precipitation; Sevag method was used to remove protein, dialysis, freezing and drying method was used to purified EPSs.The scavenging effects on·OH, DPPH radical and O 2·by EPS were investigated using spectrophotometry.The results indicated that the EPSs had strong reducing power and good scavenging ability to the ·OH and O 2·.Its ability of scavenging DPPH radical was much lower than that of VC under the same conditions.

关 键 词：假单胞菌 胞外多糖 抗氧化性 

分 类 号：R285[医药卫生—中药学]