食品过敏原羽扇豆成分的环介导等温扩增检测方法  

Detection of allergen lupin components by loop-mediated isothermal amplification

作　　者：张舒亚[1] 张坤[1] 樊彦莉 薛华杰[1] 印丽萍[1] 陈颖[2] 张璜 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]中国检验检疫科学研究院,北京100123 [3]广州迪澳生物科技有限公司,广州510663

出　　处：《食品安全质量检测学报》2014年第4期1073-1080,共8页Journal of Food Safety and Quality

基　　金：上海科委技术标准专项(12DZ0503103);出入境检验检疫行业标准制定计划项目(2012B141);上海出入境检验检疫局科研项目(HK006-2013)~~

摘　　要：目的建立食品过敏原羽扇豆成分的环介导等温扩增检测方法(loop-mediated isothermal amplification,LAMP)。方法根据羽扇豆的ITS基因设计羽扇豆的特异性引物,进行特异度、灵敏度、稳定性测试,建立LAMP检测方法。结果本文建立的食品过敏原羽扇豆成分LAMP检测方法能有效对羽扇豆成分进行快速检测,具有较强的特异度和稳定性,灵敏度可达0.001%(w:w)。结论该方法特异度强、灵敏度高,可以快速、准确检测食品中过敏原羽扇豆成分。Objective To develop a loop-mediated isothermal amplification(LAMP) for detection of allergen lupin components. Methods The primers were designed according to the ITS gene sequence and specificity, and sensitivity and stability of the LAMP detection method were analyzed. Results The de-tection method was specific and accurate, and the limit of detection was 0.001%(w:w). Conclusion The LAMP method is effective, specific and sensitive, and it is suitable for the detection of allergen lupin components in food.

关 键 词：食品过敏原 羽扇豆 环介导等温扩增 检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]