红茶多糖的体外抗氧化及糖苷酶抑制活性研究  

Studies on the in vitro antioxidant and glycosidase inhibitory effects of polysaccharides from black tea

作　　者：李淑琴[1] 陈海霞[1] 曲志爽[1] 王秀明[1] 

机构地区：[1]天津大学药物科学与技术学院,天津市现代药物传递及功能高效化重点实验室,天津300072

出　　处：《食品安全质量检测学报》2014年第6期1615-1620,共6页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31371879);国家高技术研究发展计划(863计划)项目(2013AA102207)~~

摘　　要：目的研究红茶多糖的体外抗氧化活性和降血糖活性。探讨结构修饰对红茶多糖活性的影响。方法通过还原力实验、羟基自由基清除实验、DPPH清除实验以及脂质过氧化抑制试验模型研究红茶多糖的体外抗氧化活性。通过对α-淀粉酶和α-葡萄糖苷酶的抑制实验研究红茶多糖的降血糖活性,以及对抑制α-葡萄糖苷酶动力学研究红茶多糖的抑制类型。应用超声处理、部分酸解和酶解处理三种不同的处理方式探讨结构修饰与红茶多糖活性的关系。结果在抗氧化实验中,红茶多糖活性具有良好的浓度依赖关系。在α-淀粉酶以及α-葡萄糖苷酶的抑制试验中,红茶多糖显示出一定的抑制作用。由动力学研究推测红茶多糖对a-葡萄糖苷酶的抑制作用属于非竞争性抑制。三种结构修饰方式对活性均有影响,但变化趋势与处理方式和活性模型有关。结论红茶多糖是一种良好的抗氧化剂,并具有一定的降血糖活性,相应的结构修饰可提高红茶多糖的活性。Objective To study the in vitro antioxidant activities and hypoglycemic effects of black tea polysaccharides, and to explore the structure modification effect on the activity of black tea polysaccharides. Methods In order to illustrate the antioxidant activities of black tea polysaccharides, the reducing power, scavenging activities on hydroxyl and DPPH free radicals and inhibition effects on liposome peroxidation as-says were studied. Hypoglycemic effect was also tested by the studies on inhibition effects on α-amylase andα-glucosidase. In addition, through Linerweaver-Burk plots analysis that the inhibitory type of black tea poly-saccharides effect onα-glycosidase was determined. Ultrasonic treatment, acid hydrolysis and enzymic diges-tions were acted on black tea polysaccharides to figure out the influences on activities. Results The results showed that the antioxidant activities of black tea polysaccharides had good dose-dependent manners and the inhibitory effects on α-amylase and α-glycosidase were also promising. The inhibitory type on α-glycosidase was supposed to be non-competitive inhibition. Three different processes all had affected on the activities of black tea polysaccharides, while the influence was depended on the type of process. Conclusion Black tea polysaccharide is a kind of antioxidant, and has hypoglycemic effects. Structural modifications can enhance the activity of black tea polysaccharides.

关 键 词：红茶多糖 抗氧化 降血糖 结构修饰 

分 类 号：TS201.2[轻工技术与工程—食品科学]