Effects of ubiquitin-protea-some pathway on mouse sperm capacitation, acrosome reaction and in vitro fertilization  

作　　者：WANG Hongmei SONG Changcheng DUAN Chongwen SHI Weixian LI Cunxi CHEN Dayuan WANG Yongchao 

机构地区：[1]State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100080,China [2]Institute of Cell Biology,College of Life Sciences,Beijing Normal University,Beijing 100875,China

出　　处：《Chinese Science Bulletin》2002年第2期127-132,共6页

基　　金：This work was supported by the Special Funds for Major State Basic Research Projects (Grant No. G1999053901) ; the National Natural Science Foundation of China (Grant No. 39430080).

摘　　要：Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody againstubiquitinated proteins——UCPi; the polyclonal antibodyagainst ubiquitin-anti-Ub, and a special inhibitor againstproteasome——ALLN. Furthermore, we treated the capaci-tated sperm or the eggs with these reagents separately and tested whether the normal in vitro fertilization was blocked or not. Results illustrate that UCP1, anti-Ub, and ALLN have little effects on sperm capacitation and acrosome reaction, but they do inhibit fusion of mouse sperm with eggs, which suggests that UPP play an important role in mouse in vitro fertilization.Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody against ubiquitinated proteins—UCP1; the polyclonal antibody against ubiquitin—anti-Ub, and a special inhibitor against proteasome—ALLN. Furthermore, we treated the capacitated sperm or the eggs with these reagents separately and tested whether the normal in vitro fertilization was blocked or not. Results illustrate that UCP1, anti-Ub, and ALLN have little effects on sperm capacitation and acrosome reaction, but they do inhibit fusion of mouse sperm with eggs, which suggests that UPP play an important role in mouse in vitro fertilization.

关 键 词：UBIQUITIN-PROTEASOME PATHWAY CAPACITATION ACROSOME reaction in vitro fertilization. 

分 类 号：Q492[生物学—生理学]