In vitro effects of monoclonal antibody targeted daunomycin on human gastric cancer cells  

作　　者：汪森明 陈希陶 张学庸 樊代明 

机构地区：[1]Department of Gastroenterology,Zhujiang Hospital,First Military,Medical University,Guangzhou 510282 [2]Laboratory of Gastroenterology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032

出　　处：《Journal of Medical Colleges of PLA(China)》1991年第4期324-327,331,共5页中国人民解放军军医大学学报（英文版）

摘　　要：In the present study,daunomydn （DIM） was chosen to conjugate cowdently with amonoclonal antitibody,MGb₂,against human gastric cancer cells via a cis-aconitic anhydride linker（directly） or a dextmn bridge （indirectly,） The mo-lar ratio of MGb₂ to DM in the conjugates was1:6 （direct method） and 1:54 （indirect method）,respectively.The ELISA results revealed theantibody after conjugation retained antigen-binding capacity.The conjugates showed a highly se-lective cytotoxicity to the target cells.In lh cytotoxidty test,cytotoxidty of the conjugates wasgreater than that of free DM or irrevalent conjugates to human gastric cancer cell lines,SGC-7901and very low as far as non-target cells （HeLa） were concerned.It is sugared that theselective cytotoxidty on target cells of the conjugates is mediated by the monoclonal antibody.In the present study,daunomydn (DIM) was chosen to conjugate cowdently with a monoclonal antitibody,MGb_2,against human gastric cancer cells via a cis-aconitic anhydride linker (directly) or a dextmn bridge (indirectly,) The mo-lar ratio of MGb_2 to DM in the conjugates was 1:6 (direct method) and 1:54 (indirect method),respectively.The ELISA results revealed the antibody after conjugation retained antigen-binding capacity.The conjugates showed a highly se- lective cytotoxicity to the target cells.In lh cytotoxidty test,cytotoxidty of the conjugates was greater than that of free DM or irrevalent conjugates to human gastric cancer cell lines,SGC-7901 and very low as far as non-target cells (HeLa) were concerned.It is sugared that the selective cytotoxidty on target cells of the conjugates is mediated by the monoclonal antibody.

关 键 词：STOMACH NEOPLASMS DAUNOMYCIN MONOCLONAL antibodies IMMUNOTOXIN targeting therapy 

分 类 号：R[医药卫生]