Effect of titanium dioxide nanoparticles on zebrafish embryos and developing retina  

作　　者：Ya-Jie Wang Zi-Zi He Yang-Wu Fang Yang Xu Ya-Nan Chen Guan-Qun Wang Yong-Qiang Yang Zhuo Yang Yu-Hao Li 

机构地区：[1]Key Laboratory of Tumor Microenviroment and Neurovascular Regulation,Nankai University School of Medicine [2]State Key Laboratory of Medicinal Chemical Biology,College of Chemistry,Nankai University

出　　处：《International Journal of Ophthalmology(English edition)》2014年第6期917-923,共7页国际眼科杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No.81301080);the National Key Technology R&D Program of China (2012BAI08B06);the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry

摘　　要：AIM: To investigate the impact of titanium dioxide nanoparticles(Ti O2NPs) on embryonic development and retinal neurogenesis.METHODS: The agglomeration and sedimentation of Ti O2 NPs solutions at different dilutions were observed,and the ultraviolet-visible spectra of their supernatants were measured. Zebrafish embryos were experimentally exposed to Ti O2 NPs until 72 h postfertilization(hpf). The retinal neurogenesis and distribution of the microglia were analyzed by immunohistochemistry and whole mount in situ hybridization.RESULTS: The 1 mg/L was determined to be an appropriate exposure dose. Embryos exposed to Ti O2 NPs had a normal phenotype. The neurogenesis was initiated on time, and ganglion cells, cones and rods were well differentiated at 72 hpf. The expression of fms m RNA and the 4C4 antibody, which were specific to microglia in the central nervous system(CNS), closely resembled their endogenous profile.CONCLUSION: These data demonstrate that short-term exposure to Ti O2 NPs at a low dose does not lead to delayed embryonic development or retinal neurotoxicity.AIM: To investigate the impact of titanium dioxide nanoparticles(Ti O2NPs) on embryonic development and retinal neurogenesis.METHODS: The agglomeration and sedimentation of Ti O2 NPs solutions at different dilutions were observed,and the ultraviolet-visible spectra of their supernatants were measured. Zebrafish embryos were experimentally exposed to Ti O2 NPs until 72 h postfertilization(hpf). The retinal neurogenesis and distribution of the microglia were analyzed by immunohistochemistry and whole mount in situ hybridization.RESULTS: The 1 mg/L was determined to be an appropriate exposure dose. Embryos exposed to Ti O2 NPs had a normal phenotype. The neurogenesis was initiated on time, and ganglion cells, cones and rods were well differentiated at 72 hpf. The expression of fms m RNA and the 4C4 antibody, which were specific to microglia in the central nervous system(CNS), closely resembled their endogenous profile.CONCLUSION: These data demonstrate that short-term exposure to Ti O2 NPs at a low dose does not lead to delayed embryonic development or retinal neurotoxicity.

关 键 词：titanium dioxide nanoparticles RETINA MICROGLIA ZEBRAFISH 

分 类 号：R-332[医药卫生]