木聚糖酶高产突变菌株——黑曲霉AN497的正交试验研究  

An Orthogonal Experiment on Fermentation Conditions with High Yield Xylanase Producing Strain of Aspergillus niger AN497

作　　者：白爱枝[1] 梁运章[1] 杨军[1] 李市场[2] 潘仁瑞[3] 

机构地区：[1]内蒙古大学离子束生物工程自治区重点实验室 [2]中国科学院等离子体物理研究所离子束生物工程学重点实验室,合肥230031 [3]合肥联合大学,合肥230022

出　　处：《食品与发酵工业》2004年第9期15-18,共4页Food and Fermentation Industries

基　　金：内蒙古自治区政府专项经费资助项目;国家自然科学基金资助项目 (No .10 0 6 5 0 0 1);国家"十五"科技攻关项目 (No .2 0 0 1BA30 2 8)

摘　　要：黑曲霉 (Aspergillusniger)A3经离子注入后选育出高产木聚糖酶突变株AN497,通过摇瓶液态发酵产酶培养基的L16 ( 4 4)正交实验 ,得到了优化的培养基配方 (g/L) :玉米芯粉 80 ,蔗糖 6,复合氮源 (NH4 )SO4 +NaNO3(质量比为 1∶2 ) 1 2 ,吐温 2 0 3 ,用无氮的Mandels氏无机盐溶液配制。同时对产酶菌株的发酵过程进行了试验 ,发酵 84h达产酶高峰 ,木聚糖酶活力可达 646IU/mL ,比培养基未优化前 ( 5 84 8IU/mL)提高了 1 0 5 %。AN497, a high-yielding xylanase strain, was screened by the technology of ion implantation. Through orthogonal experiment,the optimal condition was obtained for producing xylanases of Aspergillus niger AN497.The suitable medium contained(g/L): corn-core 80,cane sugar 6, complex nitragen resource(NH 4)SO 4+NaNO 3 (mass ratio 1∶2)12, Tween20 3, prepared in Mandels nutritional solution. Xylanase producing process by strain AN497 was studied, and the result showed the optimum time for xylanase secretion was about 84 ?h, the xylanase activity is as high as 646IU/mL. Xylanase activity was increased by 10. 5% compared to that of former one strain M-2980.

关 键 词：高产 突变菌株 木聚糖酶 玉米芯粉 选育 培养基配方 氮源 黑曲霉 产酶培养基 液态发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]