AS-PCR在ADH2、ALDH2基因多态型分析中的应用  

AS-PCR- a Convenient and Cost-saving Method for Analyzing the Genotypes of Alcohol Dehydrogenase 2(ADH2) and Aldehyde Dehydrogenase 2(ALDH2) Loci with High Accuracy

作　　者：曹西蓉[1] 吴德生[1] 

机构地区：[1]四川大学华西公共卫生学院,四川成都610041

出　　处：《环境与职业医学》2004年第5期371-373,共3页Journal of Environmental and Occupational Medicine

基　　金：四川省计划生育委员会基金 (编号 :98 4 1 ) ;纽约中华医学基金会

摘　　要：[目的 ]为获得更为简便经济同时精确度足够高的酒精脱氢酶 2 (ADH2 )和乙醛脱氢酶 2 (ALDH2 )基因多态型测定的新方法。 [方法 ]建立并优化等位基因特异性PCR(allelespecific PCR ,AS PCR)方法 ,通过与经典PCR RFLP方法的分型结果相比较考察其可行性。 [结果 ]AS PCR方法对 60例DNA样本的ADH2、ALDH2基因的分型结果与PCR RFLP法完全一致 ,且简便快速 ,成本低 ,特异性和准确度也足够高。 [结论 ]AS PCR方法分析ADH2、ALDH2基因多态型优点明显 。To find out a method which is more convenient,cost-saving and enough accurate to detect genotypes of alcohol dehydrogenase 2 (ADH2)and aldehyde dehydrogenase 2 (ALDH2). Establishing and optimizing the method of allele specific-PCR(AS-PCR),its detecting results of 60 DNA samples were compared with that of classical PCR-RFLP to decide the effectiveness of AS-PCR. Detecting results by PCR-RFLP and AS-PCR were thoroughly consistent. [Conclusion] Using AS-PCR to detect genotypes of ADH2 and ALDH2 is undoubtedly superior which is particularly helpful to investigations in small hospitals or on large scale.

关 键 词：AS-PCR ADH 基因多态型 分型 特异性 PCR-RFLP法 乙醛脱氢酶 应用 等位基因 成本低 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R52[医药卫生—临床医学]