海藻酸钙固定化β-半乳糖苷酶催化合成低聚半乳糖  

Synthes is of galato-oligos accharide by immobilized β-galactos idase on calcium alginate

作　　者：毛跟年[1] 范金波[1] 许牡丹[1] 张嫱[1] 檀志芬[1] 

机构地区：[1]陕西科技大学生命科学与工程技术学院,咸阳712081

出　　处：《食品科技》2004年第10期5-7,12,共4页Food Science and Technology

摘　　要：研究了强化海藻酸钙固定化β-半乳糖苷酶的方法以及固定化酶的性质,并用于制备低聚半乳糖。研究表明,用海藻酸钙包埋、戊二醛进行交联、对β-半乳糖苷酶进行固定,方法简便、酶的活力回收率高。所得固定化酶强度和活力高,对热、pH值耐受范围较游离酶宽,最佳反应温度和pH与游离酶相同,且贮存稳定性好。以乳糖为原料,用海藻酸钙固定化β-半乳糖苷酶催化合成低聚半乳糖,随着时间的延长,低聚半乳糖合成率呈抛物线变化。在温度55℃、pH6.0、乳糖浓度40%、反应时间为30h时,低聚半乳糖的合成率达最大值36.37%。In this paper,tos idase was also inv es tigated.Calcium alginate as immobiliz ed enz yme s upporters and glutaraldehy de as cross linking agent were introduced to prepare the immobiliz ederimen t res ults have s howed that calcium alginate as immobiliz ed enz yme s upporter s is very good and the optimum temperature,pH and s ubs trate concentration were 55℃,6.0,40%res pectively.The yield of galacto-oligos accharides us ing immobili zedactos idase to hydrolyze lactose reached36.37%.

关 键 词：Β-半乳糖苷酶 固定化 海藻酸钙 低聚半乳糖 

分 类 号：TS201.25[轻工技术与工程—食品科学]