补料分批培养生产2-酮基-D-葡萄糖酸的研究  

Studies on the Fermentative Production of 2-Keto-D-gluconic Acid by Pseudomonas fluorescens in Fed-batch Culture

作　　者：孙文敬[1] 赵峰梅[2] 杨庆文[2] 郭金权[2] 秦丽 刘敬泽[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]山西省生物研究所,山西太原030006 [3]郑州市生物化工厂,河南郑州450007

出　　处：《食品科学》2004年第11期115-117,共3页Food Science

摘　　要：研究了补糖对荧光假单胞菌(Pseudomonas fluorescens)AR4生产2-酮基-D-葡萄糖酸的影响,并在50kL发酵罐上进行了补料分批培养工业应用性试验。研究结果表明,采用补料分批培养方法,能有效提高发酵液中的产物浓度;开始补糖时发酵液中的残糖浓度以3%～6%为宜;补料分批培养方法及AR4菌株可用于2-酮基-D-葡萄糖酸的工业化规模生产中。The effects of feeding glucose on the production of 2-keto-D-gluconic acid by Pseudomonas fluorescens AR4 werestudied, and the application of fed-batch culture in the industrial production was also investigated in a 50kL fermentor. The resultsshowed that the concentration of the product could be increased effectively in fed-batch culture, the suitable concentration ofresidual glucose in the fermentation broth was 3%～6% while beginning to feed glucose, and the culture method and the strainAR4 could be applied in the industrial fermentation process.

关 键 词：补料分批培养 2-酮基-D-葡萄糖酸 发酵液 发酵罐 补糖 工业应用 研究结果 养生 AR 糖浓度 

分 类 号：TS202.3[轻工技术与工程—食品科学] TQ463.4[轻工技术与工程—食品科学与工程]