金针菇菌丝体深层培养工艺研究  

Submerged Culture Production of Flammulina velutipus Mycelium

作　　者：方海洲[1] 梁世中[1] 陈碧坚[1] 高孔荣[1] 

机构地区：[1]华南理工大学生物工程研究所

出　　处：《生物工程学报》1993年第4期379-382,共4页Chinese Journal of Biotechnology

摘　　要：在１．８Ｌ气升式发酵反应器中，研究了金针菇菌丝体深层培养工艺条件及其特性。结果表明：金针菇菌丝体深层培养可用采用玉米，黄豆和糖蜜等廉价原料作主要碳氮源，最适需氧量为１．０－２．０×１．０＾－７ｍｏｌ Ｏ２／ｍｌ·ｍｉｎ·ａｔｍ；培养２４－７２ｈ内，菌丝球生长循立方根规律，即ｘ＾１／３＝０．７８６＋０．０２７ｔ。在最佳发酵条件下，摇瓶培养４天，菌丝干重达２４．４ｍｇ／ｍｌ；在反应器培养４天。Flammulina velutipus was cultivated in a 1.8 liter air-lift fermentor. The result showed that corn, soybean and molasses were the best economical carbon and nitrogen sources for the culture. The optimum oxygen requirement was 1.0—2.0×10^(-7)mol O_2/ml·min·atm. Mycelial growth follows cubic root law, i.e., x^(1/3)=0.786+0.027t. Under the optimum conditions, the mycelial dry weight yields for 4 days were 24.4g/L in shaking culture and 25.7g/L in air-lift fermentor cultivation.

关 键 词：金针菇 深层培养 菌丝体 发酵 

分 类 号：S188[农业科学—农业基础科学]