三种试验方法检测食品中单核细胞增多症李氏菌的研究  

Comparison of PCR,Biotinylated Probe Labeled by PCR and culture Methods for Detection of L. monocytogenes in Food

作　　者：杨百亮[1] 马梅利 李绪英[1] 周志江[2] 徐克诚[2] 谭小海[2] 胡咏梅[2] 丁延令 

机构地区：[1]山西农业大学动医系,030801 [2]中国人民解放军兽医大学 [3]长春市动物检疫站l

出　　处：《食品科学》1993年第9期61-63,共3页Food Science

摘　　要：采用PCR、PCR制备生物素探针和分离培养法等3种试验方法,分别对采自长春市某市场68份零售猪肉和70份牛奶进行了检测。结果 PCR呈现阳性41份;探针呈现阳性33份;分离培养呈现阳性22份,3种试验方法的阳性率之比为100.00 :73.32:48.88。对单核细胞增多症李氏菌、其它种李氏菌和细菌检测的结果表明,PCR及其制备的探针具有高度种特异.PCR敏感性高,快速简便,但对于大批样品的检测,探针则具有操作简便和敏感的优点。This paper reports the application of PCR, Biotinylated probe labeled by PCR and culture methods for detection of L. monocytogenes in 68 pork samples and 70 milk samples collected from some free markets in Changchun city. The sensitivity of the above three tests have been compared. There are 41 positive samples in PCR test; 33 positive samples in dot-blot test and 22 positive samples in culture method. The ratio of the positive rate of the above three tests was as following: 100. 00 : 73. 32 : 48. 88. PCR reacted positively to all strains of L. monocytogenes , but negatively to other species of Listeria and bacteria. It is concluded that PCR is the most sensitive , simple and rapid method for detection of L. Monocytogenes in food , but for large quantity of samples , dot-blot test with the probe may be helpful.

关 键 词：食品 单核细胞 增多症 李氏菌 检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]