检测有机磷农药残留生物传感器的温度特性  

Temperature property of thermal biosensor used in organophosphorous pesticide detection

作　　者：郑艺华[1] 王芳芳[1] 许学勤[1] 徐斐[1] 华泽钊[1] 

机构地区：[1]上海理工大学食品科学与工程研究所,上海200093

出　　处：《农业工程学报》2005年第1期132-135,共4页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金资助项目(50176032);上海市科技兴农重点攻关项目(2001 -4)

摘　　要：为了考察检测有机磷农药残留用量热式生物传感器的温度特性,本文采用分光光度法研究了固定化鸡肝酯酶的活力、农药敌敌畏对鸡肝酯酶的抑制等与温度的关系。在生物传感器的实测条件下,研究了不同温度固定化酶活力的操作流失情况。研究显示,温度对酶反应的影响很大,控温精度的提高将增强检测的精度和稳定性。温度55～60℃时,是酶反应速度最快的区域;抑制时间5min时,农药敌敌畏对鸡肝脂酶产生了明显的抑制作用。在农药浓度小于1mg/L的区域,随浓度变化的相对抑制关系接近于线性;一定浓度的农药敌敌畏对酶的抑制程度随着温度的变化不剧烈,在50℃左右时抑制程度较大;通过对流出液酶活的测定,发现温度没有对酶活的操作流失产生明显的影响。综合酶活力和热量损失,选定最适检测温度为40℃。In order to investigate temperature property for thermal biosensor used in organophosphorous pesticide detection, the immobilized chicken liver-esterase activity and inhibition ability by dichlorvos at different temperatures were studied by using spectrophotometer. Under the real test condition, operational loss of immobilized chicken liver-esterase was observed. The result is shown as follows: enzyme reaction is affected by temperature significantly and the esterase activity reaches highest at 55 - 60°C; when inhibition reaction time is set to 5 minutes, inhibition degree increases with pesticide concentration. The inhibition curve is close to linear at lower concentrations; the inhibition ability by dichlorvos is not acutely affected with temperature, and it reaches maximum at 50°C; it is found that operational loss of the chicken liver-esterase have similar values at different temperature. The optimum temperature of 40°C is selected for achieving maximum reaction rate and minimum heat damage.

关 键 词：鸡肝酯酶 量热式生物传感器 检测温度 固定化酶 

分 类 号：TS207.53[轻工技术与工程—食品科学]