双水相系统中酶法水解明胶制取氨基酸的研究  

Study on Aqueous Two-phase Systems to Produce Amino Acids Through Gelatin Hydrolysis

作　　者：张刚[1] 黄登禹[1] 张丛越[1] 

机构地区：[1]天津市工业微生物研究所,天津300221

出　　处：《食品科学》2005年第1期121-124,共4页Food Science

基　　金：天津市科学基金资助项目(963706111)

摘　　要：在PEG—盐双水相系统中,进行了蛋白酶催化明胶水解制取混合氨基酸的研究。双水相系统组成为20%PEG1540、10%磷酸钾盐、pH7.5时,加入3%碱性蛋白酶发酵液和35%明胶,45℃下水解10h,转化率达到83.2%。酶分配于顶相,水解产物分配于底相,混合氨基酸的总收率为76.7%。水解产物中混合氨基酸由13种氨基酸组成,其中7种为必须氨基酸。s:To produce amino-acids through gelatin hydrolysis, catalyzed by protease by aqueous two -phase systems was studied. The constituted 20% PEG1540 with 10% potassium phosphate by the aqueous two phase system with 3% alkali protease and 35% gelatin added was hydrolyzed at the conditions of pH7.5 and 45℃ for 10h. With the hydrolysis of 83.2%, 76. 7% amino acids was obtained, totally constituted 13 kinds of amino acids.

关 键 词：双水相系统 明胶 水解 氨基酸 

分 类 号：TQ925.1[轻工技术与工程—发酵工程] TQ028.4