转SCK基因大米的营养学评价—小型猪比较喂养研究  

THE NUTRITIONAL EVALUATION OF GENETICALLY MODIFIED RICE—COMPARATIVE FEEDING STUDY OF MINIPIGS

作　　者：杨月欣[1] 陈淑蓉[1] 韩军花[1] 杨晶明[1] 杨晓光[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《营养学报》2005年第1期38-41,45,共5页Acta Nutrimenta Sinica

基　　金：科技部国家转基因植物研究与产业化专项(No.J00-003);法国达能膳食营养与宣教基金(No.DIC2002-02)

摘　　要：目的:通过小型猪喂养实验,分析和评价转豇豆胰蛋白酶抑制剂基因大米(转SCK基因大米)的营养价值,并比较与亲本大米之间的差异。方法:选择断乳期雄性中国实验用小型猪15只,初始体重(6.22±0.42)kg,按体重和窝别随机等分成3组,分别为普通饲料组(NG),仅饲喂市售猪饲料;对照大米组(CG),饲喂70%亲本大米+基础饲料;转基因大米组(GG),饲喂70%转SCK基因大米+基础饲料。基础饲料包括12%小麦麸,8%豆粕,6%酪蛋白和4%的矿物质和维生素混合物。喂养62d,观察和比较三组动物生长发育、摄食量、血生化指标、骨骼发育、组织病理等方面的差异。结果:GG组动物与CG组的一般营养状况、血常规和血生化值、体格发育、骨骼与肌肉发育和脏器发育及病理方面均未见明显差异,其营养作用基本等同,且两个大米组动物的体重增长值均高于NG组(P<0.05)。GG和CG组动物的食物利用率相近,但均高于NG组(P<0.05)。结论:转SCK基因大米与对照大米对小型猪的生长发育的影响基本等同,未发现转SCK基因大米造成任何可观察到的非预期不良结果。Objective: To assess the nutritional value of rice genetically modified with SCK gene by feeding minipigs and compare with parental rice. Methods: 15 weaning male minipigs weighted 6.22±0.42kg were divided into three groups: (1) Normal group (NG), fed only a commercial pig diet, (2) Control or parental rice group ( CG), fed 70% parental rice + 30% basic diet, (3) GM rice group (GG), fed 70% GM rice + 30% basic diet. The basic diet includes 12% wheat bran,8% bean,6% casein,and 4% mixture of vitamins and mineral elements. All minipigs were housed individually in the pen, and had ad libitum access to feed and water. The feeding period was 62 days, the body growth (weight, height, body length, breast circumference etc ) and feed intake of animals were recorded. All animals were sacrificed after the experiment and the blood chemical analysis, bone and organs development, histological examination were conducted. Results:Throughout the 62 days feeding period, the minipigs in each group had no abnormal signs and grew well. The growth, hematological and blood chemical analysis, organ weight, bone measurement showed no significant difference between GG and CG group. The cumulative weight gain and gain/feed of minipigs in CG and GG groups were higher than NG group (P<0.05). Conclusions: The feeding value of GM rice and parental rice were similar in this study, and no detrimental unexpected effects were observed in rice genetically modified with SCK gene.

关 键 词：转基因大米 营养学评价 小型猪喂养 

分 类 号：R151[医药卫生—营养与食品卫生学]