羊水细胞培养诊断染色体病  

Amniotic Fluid Cell Culture in Prenatal Diagnosis of Chromosomal Abnormalities

作　　者：王世雄[1] 胡琴[1] 李一峰[1] 曹英[1] 黄荣魁[1] 

机构地区：[1]上海第二医科大学附属新华医院,上海市儿科医学研究所

出　　处：《上海第二医科大学学报》1994年第2期98-101,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市科委资助研究课题

摘　　要：１９８８年５月至１９９３年４月，对２０８例妊娠１６～２０周孕妇进行羊膜腔穿刺，取羊水细胞培养作染色体病产前诊断，检出异常６例，检出率２９％。６例异常包括：在９例夫妇一方为染色体平衡易位携带者对象中发现２例；８０例高龄（＞３５岁）孕妇中证实２例；１例为怀疑患血友病Ａ的男胎；另一例发现在一位情绪极其低落的孕妇中。采用改良的羊水细胞培养方法，各种染色体显带技术，羊水细胞培养成功率大大提高，成功率为９９．５％。进一步缩短了培养时间，平均７．６ｄ。４例严重红细胞污染（每ｍｌ羊水的红细胞污染数＞７．７×１０７）的羊水细胞培养都获得成功。Amniocentesis was carried out in 203 cases of pregnancy during 16-20 GW for prenatal diagnosis of chromosomal abnomalities. Abnormal karyotypes were detected in 6 cases(2.9%). The risk of fetus afflicted born of one of the parents being carrier of balanced translocation was 2 out of 9.Out of 80 cases of advanced maternal age(>35a), 2 exhibited chromosomal abnormalities.A male fetus was suspected of having hemophilia A. Another abnormal fetus was born of a pregnant woman with great anxiety. Using the improved method of amniotic fluid cultivation and various techniques of chromosome banding, the rate of success was greatly increased, approaching 99.5%, and the duration of culturing much shortened,averaging 7.6d.In 4 cases, even though the amniotic fluid was heavily contaminated with RBC(>7.7×107/ml),the culturing results were still satisfactory.

关 键 词：羊水 细胞培养 产前诊断 染色体病 

分 类 号：R714.55[医药卫生—妇产科学]