低双乙酰啤酒酵母激光诱变条件的研究  

Study on laser induced mutation condition of low-diacetyl producing Saccharomyces cerevisiae

作　　者：张智维[1] 张璐[1] 代春吉[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西咸阳712081

出　　处：《中国酿造》2005年第3期27-28,共2页China Brewing

摘　　要：以激光为诱变剂照射啤酒酵母,得到1株双乙酰值低的啤酒酵母。用该菌株酿造的啤酒,发酵液中双乙酰含量为0.0836mg/L,比用原菌株酿造的啤酒下降了53%。激光诱变的条件为:波长623.8nm(红色)、功率6mW的He-Ne激光,用滤纸片法照射啤酒酵母,照射时间为5min。A low-diacetyl producing Saccharomyces cerevisiae was obtained by using laser as a mutagen to irradiate S. cerevisiae. The fermented broth of the beer made from this strain contained diacetyl of 0.0836 mg/ L, a 53% reduction than the beer made from original strain. The mutation condition was: 623.8 nm (red) wavelength, He-Ne laser with 6 mW power, filter-paper irradiation method, and 5 min irradiation time.

关 键 词：激光诱变 双乙酰 啤酒酵母 

分 类 号：Q813.5[生物学—生物工程]