高效液相色谱法测定焦糖色素中4-甲基咪唑  

DETERMINATION OF 4-METHYL INIDAZOLE IN CARAMEL BY HPLC.

作　　者：肖义夫[1] 廖百森[1] 

机构地区：[1]重庆市疾病预防控制中心,重庆400042

出　　处：《现代预防医学》2005年第3期249-250,共2页Modern Preventive Medicine

摘　　要：目的 :建立 4 -甲基咪唑的快速定量测定方法。方法 :先用有机溶剂将焦糖色素中 4 -甲基咪唑提取出来 ,再经过显色后采用高效液相色谱法测定。结果 :该法的平均回收率为 91% ,相对测量不确定度小于 5 %。结论 :该方法能有效去除其他组分的干扰 ,并具有较高的准确度和可靠性 ,可对 4 -甲基咪唑进行快速测定。Objective:To develop the method for the determination o f 4-methyl imid azole in caramel.Method:The 4-methyl imidazole was separated fro m caramel by organic solvent,and be coloured,then be identified by HPLC. Results:The ave rage recovery w as 91% and relative uncertainty of measurement was below 5%.Conclusions :Determination of 4-methyl imidazole by HPLC could remove interference,a nd was rapid,reliable and accurate.

关 键 词：高效液相色谱法 焦糖色素 4-甲基咪唑 含量测定 

分 类 号：R155.5[医药卫生—营养与食品卫生学]