花生蛋白碱性蛋白酶水解物对血管紧张素转化酶抑制作用的初步研究(英文)  

Angiotensin I-converting Enzyme Inhibitory Activity of Peanut Protein Hydrolysates Prepared with Alcalase

作　　者：刘焕[1] 黎观红[1] 施用晖[1] 乐国伟[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《花生学报》2005年第1期8-14,共7页Journal of Peanut Science

基　　金：中央级科研院所科技基础性工作专项基金重点项目资助(No.2001DEA20022)

摘　　要：分别以碱性蛋白酶Alcalase和中性蛋白酶Neutrase对花生分离蛋白进行水解,制备花生分离蛋白水解物,并测定不同水解时间所得产物对血管紧张素转化酶(ACE)的抑制活性。未水解的花生分离蛋白没有 ACE抑制活性,用中性蛋白酶Neutrase水解所得的水解物显示弱ACE抑制活性。然而,碱性蛋白酶Alcalase水解物具有很强的ACE抑制活性,水解0.5 h时水解物活性最高,其半抑制浓度为(IC50)0.56 mg/mL。本研究表明,当用碱性蛋白酶Alcalase水解时,花生分离蛋白是生产ACE抑制肽的良好蛋白质来源,花生分离蛋白碱性蛋白酶 Alcalase水解物可作为具有降压功能的功能食品添料。Peanut protein hydrolysates were prepared by enzymatic hydrolysis with Alcalase and Neutrase, and the angiotensin I-converting enzyme (ACE) inhibitory activities of the enzymatic hydrolysates were investigated at different hydrolysis times. The unhydrolyzed protein showed no inhibitory activity. Hydrolysates generated with Neutrase displayed very low ACE inhibitory activity, while those obtained with Alcalase exhibited high inhibitory activity. The highest ACE inhibitory activity with the IC_ 50 value of 0.56 mg protein/mL was found in the hydrolysate obtained with Alcalase at 30 min of hydrolysis time. These results indicate that peanut protein is a good protein source of ACE inhibitory peptides when hydrolyzed with the protease Alcalase. The peanut protein hydrolysates prepared with Alcalase might be utilized for physiologically functional foods with antihypertensive activity.

关 键 词：碱性蛋白酶 花生蛋白 蛋白水解物 酶水解 中性蛋白酶 ACE抑制活性 降压功能 血管紧张素转化酶 抑制作用 分离 

分 类 号：S565.2[农业科学—作物学] TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]