固定化酶生产低聚异麦芽糖工艺研究  

Technique of Isomaltooligosaccharide Preparation with Immobilizing Enzymes

作　　者：吴定[1] 邹耀洪[2] 王云[3] 陈爱英[2] 姚明兰[1] 

机构地区：[1]南京财经大学食品科学与工程学院,江苏省农产品深加工与品质控制重点实验室,江苏南京210003 [2]常熟理工学院生化系,江苏常熟215500 [3]南京农业大学生命科学院,江苏南京210095

出　　处：《食品科学》2005年第3期125-127,共3页Food Science

基　　金：江苏省教育厅自然科学基金项目(01KJD150006)

摘　　要：壳聚糖溶解于20%的盐酸,配成25%的壳聚糖溶液,然后用注射器注射到含15%氢氧化钠和30%甲醇的混合溶液中凝结成2mm左右的中空球形壳聚糖。经4%的戊二醛活化的中空球形壳聚糖分别与α-葡萄糖转苷酶、α-淀粉酶、β-淀粉酶、切枝普鲁兰酶在室温反应2h,4℃静置过夜,制备固定化酶。固定化酶的最适pH值约降低1个单位,最适温度提高约10℃。固定化α-淀粉酶和β-淀粉酶的相对酶活力分别为7.2%和22.3%。四种不同的固定化酶重组构成酶催化反应器,生产低聚异麦芽糖含量达38.9%。The hollow globular chitosan was made by dissolving in 20% HCl first and then injecting 25% chitosan solution into 15% NaOH and 30% CH3OH mixture and coagulating into 2mm hollow globular chitosan. The hollow globular chitosan was treated with 4% gluluraldehyde, then immobilized withα-amylase, β-amylase and Brulan enzyme at 25℃ for 2h, respectively. The best reaction pH of the immobilized enzymes decreased about 1 unit, the reaction temperature of the immobilizing enzymes increased about 10℃.The relative activity of enzyme on the immobilized α-amylase and the immobilized β-amylase became 7.2% and 22.3%, respectively. The content of isomaltooligosaccharide reached 38.9% in the bioreactor.

关 键 词：低聚异麦芽糖 固定化酶 中空壳聚糖 

分 类 号：Q814[生物学—生物工程]