食物中维生素C的流动注射分析法  

DETERMINATION OF TOTAL VITAMIN C IN FOODS BY FLOW INJECTION ANALYSIS

作　　者：赵云峰[1] 王光亚[1] 徐嵘[1] 贾毓 

机构地区：[1]中国预防医学科学院营养与食品卫生研究所

出　　处：《营养学报》1994年第2期186-191,共6页Acta Nutrimenta Sinica

摘　　要：本文介绍了测定食物样品中维生素Ｃ（ＶＣ）的一种自动分析方法一流动注射分析法。用本方法测定了一些蔬菜和水果样品中ＶＣ的含量。结果表明，ＶＣ的标准溶液的直线回归方程为：Ｙ＝一０．００４＋ｌ，５３５×ｌ０－６ｘ（ｒ＝０．９９９５）。本方法线性好，线性范围在０．０２２７～０．２２７ｍｍｏｌ／Ｌ之间，最低检出量为０．０８μｇ。以标准参考物测定的回收率在９０％～１１０％之间，变异系数＜１０％；本方法测定结果与标准荧光法进行了比较，结果一致。本方法系自动化测定方法，操作简便，适用于测定食物中的ＶＣ。flow injection system has been introduced to automate the analysis oftotal vitamin C(VC)in foods.The VC contents of vegetables and fruitswere determined.The results were comparable with those from a standardfluorometric method.The regression equation of calibration curve was Y=一0.004+ 1.535x,r=0.9995.A good linear relation was obtained in therange of 0.0227一0.227mmol/L.The absolute detection limit was 0.08μg.Therecoveries were 91%一110%,The coefficient of variation was<10%,Thismethod was automatic and the procedure wa:simple and suitable for deter-mination of VC in foods.

关 键 词：维生素C 流动注射分析法 食物 

分 类 号：R151.3[医药卫生—营养与食品卫生学]