正交试验优化绿茶中茶多糖醇析水提法的实验研究  

Experimental Studies on the Extract Technology of Tea Polysaccharide from Green Tea by Orthogonal Test

作　　者：许旋[1] 骆晓枫[2] 陈兆星[1] 李勤瑜[1] 陈剑经[2] 罗一帆[2] 

机构地区：[1]华南师范大学化学系,广州510631 [2]中山大学中山医学院,广州510080

出　　处：《中药材》2005年第4期327-329,共3页Journal of Chinese Medicinal Materials

基　　金：国家重点基础研究"973计划"项目 (G1998051201 );广东省科技计划项目(2003C20405);农业部茶叶化学工程重点开放实验室开放课题(200401)

摘　　要：采用正交试验法对绿茶中茶多糖的醇析水提法的影响因素进行了探讨,应用正交设计、方差分析综合讨论了茶多糖提取的产率、纯度和成本的影响工艺条件,最后确定提取茶多糖的工艺条件为:沸水浸提时间为30min,等量氯仿萃取次数为3次,等量乙酸乙酯萃取次数为1次,沉析用的乙醇浓度为60%。本文的实验结果为改善茶多糖的提取方法提供了实验基础。The effect factor of extraction of tea polysaccharide was investigated with orthogonal test. The effects of extraction conditions on yield, purity of tea polysaccharide and cost of extraction were discussed. The results show that the optimum conditions for extracting tea polysaccharide from green tea are as follows: extraction time is 30 minutes with water at 95℃,extraction times with chloroform is 3, extraction times with CH_3COOC_2H_5 is 1 and concentration of alcohol is 60%.

关 键 词：水提法 实验研究 绿茶 糖醇 优化 乙酸乙酯萃取 工艺条件 正交试验法 茶多糖 正交设计 多糖提取 综合讨论 方差分析 氯仿萃取 浸提时间 乙醇浓度 提取方法 次数 等量 

分 类 号：R284.2[医药卫生—中药学] R730.1[医药卫生—中医学]