微胶囊化提高生物传感器中固定化鸡肝酶操作稳定性的研究  

Study on Biosensor Encapsulation for Improving the Operational Stability of Immobilized Chicken Liver-esterase

作　　者：郑艺华[1] 王芳芳[1] 徐斐[1] 许学勤[1] 华泽钊[1] 

机构地区：[1]上海理工大学食品科学与工程研究所,上海200093

出　　处：《食品科学》2005年第4期32-36,共5页Food Science

基　　金：国家自然科学基金资助项目(50176032);上海市科技兴农重点攻关项目(2001II-4);上海市教委青年基金项目(02GQ21);上海市科委启明星计划资助项目(02QF14030)

摘　　要：离子交换吸附固定化酶方法,简单、便宜,但稳定性较差。为了提高采用离子交换树脂固定化酶生物传感器的操作稳定性,在保证较小酶活损失的前提下,利用海藻酸盐对固定化鸡肝酶进行微胶囊化,研究了不同浓度海藻酸钠和氯化钙溶液等条件对酶活保留率的影响。在生物传感器实际操作条件下,对微胶囊化前后的固定化酶活保留率进行了比较。结果表明,使用质量比0.2%的海藻酸钠和0.1mol/L的氯化钙溶液进行微胶囊化并静置30min时,操作稳定性得到了明显的提高,同时固定化酶活力损失较少。另外,采用在Tris缓冲液中加入0.025mol/L钙离子的方式对防止胶囊的破坏进行了尝试,这进一步提高了稳定性。Immobilizing enzyme by ion exchange adsorption is easy and inexpensive, but its stability is poor. For improving operational stability of immobilized enzyme by biosensor encapsulation resin the chicken liver-esterase was encapsulated by alginate. Influences on activity rate of enzyme in different SA (Sodium alginate) concentrations, CaCl2 (calcium chloride) concentration and other operational conditions have been investigated. Activity rate of immobilized enzyme was compared with the entrapped calcium alginate under real condition. The results showed that the operational stability was obviously enhanced in 0.2% SA, 0.1mol/L CaCl2 and 30 minutes standing. Moreover, attempt of adding 0.025mol/L Ca+ into Tris buffer would also improve the stability of alginate gel.

关 键 词：操作稳定性 固定化酶 微胶囊化 离了交换吸附法 

分 类 号：Q556[生物学—生物化学]