超高压灭活枯草芽孢杆菌(AS1.140)的参数优化  

Optimization of ultra high pressure processing parameters on survivors of Bacillus subtilis AS 1.140

作　　者：曾庆梅[1] 潘见[1] 谢慧明[1] 杨毅[1] 徐慧群[2] 黄训端[1] 赵阳楠[1] 

机构地区：[1]合肥工业大学农产品生物化工教育部重点实验室,合肥230069 [2]安徽省出入境检验检疫局,合肥230001

出　　处：《农业工程学报》2005年第4期158-162,共5页Transactions of the Chinese Society of Agricultural Engineering

基　　金：安徽省教育厅重点科研项目(2004kj004zd);农产品生物化工教育部重点实验室开放基金(NKF2004005)

摘　　要：该文采用响应曲面方法中的Box-Behnken模式,对超高压灭活枯草芽孢杆菌进行了试验优化设计,并进行了实验分析验证。试验结果表明:压力、温度、保压时间是超高压灭活枯草芽孢杆菌的显著影响因子,分析表明其显著度顺序为压力>温度>保压时间;在本试验条件范围内建立并验证的超高压杀灭枯草芽孢杆菌的回归模型准确有效;优化得出10组杀灭106cfu/mL枯草芽孢杆菌工艺参数的取值范围为压力343.79～475.75MPa,温度27.47～57.44℃,保压时间14.14～19.72min。Ultra high pressure processing (UHPP) to Bacillus subtilis was investigated, and all experiments were designed and optimized with Box-Behnken concept of response surface methodology (RSM). Results show that the pressure, temperature and dwell time are the significant factors for ultra high pressure sterilization to Bacillus subtilis, and the significant order is as follows: pressure > temperature > dwell time. The regression equation (model) for UHPP to Bacillus subtilis was established, and also verified with the check experiments. The optimized processing parameters of 10 groups were obtained, and the scales of variable factors to sterilize Bacillus subtilis of 106 cfu/mL are as follows: pressure 343.79-475.75 MPa, temperature 27.47-57.44°C and dwell time 14.14-19.72 min.

关 键 词：超高压 枯草芽孢杆菌 响应曲面法 优化 

分 类 号：TS201[轻工技术与工程—食品科学] Q932[轻工技术与工程—食品科学与工程]