女性阴道中产细菌素的乳杆菌筛选与鉴定  

Screening and identification the Lactobacillus producing bacteriocin in women vagina

作　　者：赵慧[1] 薛平海[1] 宫正[1] 张德民[1] 曹文伟[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《中国微生态学杂志》2005年第3期172-173,共2页Chinese Journal of Microecology

摘　　要：目的对从60例健康女性阴道中筛选出产生细菌素的优势乳杆菌进行鉴定,并为研制开发微生态制剂提供优良可靠菌种。方法利用牛津杯法筛选出19株乳杆菌,其菌株发酵乳酸量高并且产生细菌素。对19株乳杆菌进行了多项理化鉴定。结果19株乳杆菌分别为格氏乳杆菌9株,唾液乳杆菌1株,卷曲乳杆菌9株。结论筛选的19株乳杆菌是健康女性阴道中的优势有益菌,具有较强的产酸能力,都产生细菌素,其中16株产生过氧化氢,某些菌株具有较高的生产应用价值。Objective Identifying the dominant Lactobacillus producing bacteriocin,which were screened from 60 healthy women vaginas,provides choice and credible strains for the research of probiotic.Methods 19 strains of Lactobacillus which produce bacteriocin and abundant lactic acid were screened by the method of Oxford-cup tests,and they were identified by several important biochemical characteristics.Results According to the results of these experiments,19 strains were identified that 9 were Lactobacillus.gasseri,1 was Lactobacillus.salivarius and 9 were Lactobacillus.crispatus.Conclusions These 19 strains Lactobacillus which were screened through this experiment were dominant beneficial bacteria in the healthy female vagina,which had high ability of producing acid and bacteriocin.16 strains of them produce H_2O_2 and some had great probiotic production value.

关 键 词：乳杆菌 细菌素 筛选 鉴定 阴道 女性 微生态制剂 研制开发 牛津杯法 产酸能力 过氧化氢 应用价值 发酵乳 有益菌 优势 健康 菌株 可靠 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] TS202.3[轻工技术与工程—食品科学与工程]