Molecular Cloning of a Novel Mouse Testis-specific Spermatogenic Cell Apoptosis Inhibitor Gene mTSARG7 as a Candidate Oncogene  

作　　者：Xiao-JunTAN Xiao-WeiXING Lu-YunLI Zhao-DiWU Chang-GaoZHONG Dong-SongNIE Jun-JiangFU YangXIANG YunDENG Guang-XiuLU 

机构地区：[1]InstituteofHumanReproductiveandStemcellEngineering,CentralSouthUniversity,Changsha410078,China

出　　处：《Acta Biochimica et Biophysica Sinica》2005年第6期396-405,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(No.30371493,No.G1999055901);the Major State Basic Research Development Program of China(No.G1999055901)

摘　　要：A novel mouse gene,mTSARG7(GenBank accession No.AY489184),with a full cDNA length of 2279 bp and containing 12 exons and 11 introns,was cloned from a mouse expressed sequence tag (GenBank accession No.BE644543)that was significantly up-regulated in cryptorchidism.The gene was located in mouse chromosome 8A1.3 and encoded a protein containing 403 amino acid residues that was a new member of the acyltransferase family because the sequence contained the highly conserved phosphate acyltransferase(PlsC)domain existing in all acyltransferase-like proteins.The mTSARG7 protein and AU041707 protein shared 83.9% identity in 402 amino acid residues.Expression of the mTSARG7 gene was restricted to the mouse testis.The results of the in situ hybridization analysis revealed that the mTSARG7 mRNA was expressed in mouse spermatogonia and spermatocytes.Subcellular localization studies showed that the EGFP- tagged mTSARG7 protein was localized in the cytoplasm of GC-1 spg cells.The mTSARG7 mRNA expres- sion was initiated in the mouse testis in the second week after birth,and the expression level increased steadily with spermatogenesis and sexual maturation of the mouse.The results of the heat stress experiment showed that the mTSARG7 mRNA expression gradually decreased as the heating duration increased.The pcDNA3.1 Hygro(-)/mTSARG7 plasmid was constructed and introduced into GC-1 spg cells by liposome transfection.The mTSARG7 can accelerate GC-1 spg cells,causing them to traverse the S-phase and enter the G2-phase,compared with the control group where this did not occur as there was no transfection of mTSARG7.In conclusion,our results suggest that this gene may play an important role in spermatogenesis and the development of cryptorchid testes,and is a testis-specific apoptosis candidate oncogene.A novel mouse gene,mTSARG7(GenBank accession No.AY489184),with a full cDNA length of 2279 bp and containing 12 exons and 11 introns,was cloned from a mouse expressed sequence tag (GenBank accession No.BE644543)that was significantly up-regulated in cryptorchidism.The gene was located in mouse chromosome 8A1.3 and encoded a protein containing 403 amino acid residues that was a new member of the acyltransferase family because the sequence contained the highly conserved phosphate acyltransferase(PlsC)domain existing in all acyltransferase-like proteins.The mTSARG7 protein and AU041707 protein shared 83.9% identity in 402 amino acid residues.Expression of the mTSARG7 gene was restricted to the mouse testis.The results of the in situ hybridization analysis revealed that the mTSARG7 mRNA was expressed in mouse spermatogonia and spermatocytes.Subcellular localization studies showed that the EGFP- tagged mTSARG7 protein was localized in the cytoplasm of GC-1 spg cells.The mTSARG7 mRNA expres- sion was initiated in the mouse testis in the second week after birth,and the expression level increased steadily with spermatogenesis and sexual maturation of the mouse.The results of the heat stress experiment showed that the mTSARG7 mRNA expression gradually decreased as the heating duration increased.The pcDNA3.1 Hygro(-)/mTSARG7 plasmid was constructed and introduced into GC-1 spg cells by liposome transfection.The mTSARG7 can accelerate GC-1 spg cells,causing them to traverse the S-phase and enter the G2-phase,compared with the control group where this did not occur as there was no transfection of mTSARG7.In conclusion,our results suggest that this gene may play an important role in spermatogenesis and the development of cryptorchid testes,and is a testis-specific apoptosis candidate oncogene.

关 键 词：mTSARG7 TESTIS SPERMATOGENESIS ONCOGENE 

分 类 号：R730.2[医药卫生—肿瘤] Q785[医药卫生—临床医学]