不同培养料的双孢蘑菇多酚氧化酶活性抑制研究  

Studies on Inhibition of Polyphenoloxidase(PPO) in Agaricus bisporus Cultured by Different Composts

作　　者：谢雯君[1] 林启训[1] 陈汇川[1] 钟建业[1] 陈强[1] 王则金[1] 

机构地区：[1]福建农林大学食品科学学院,福州金山350002

出　　处：《中国农学通报》2005年第7期94-97,348,共5页Chinese Agricultural Science Bulletin

基　　金：福建省产学研联合开发基金项目"食用菌气调冷藏保鲜技术的研究与开发"(闽产学研[2003]02号)。

摘　　要：为解决双孢蘑菇保鲜和加工中的褐变问题,以邻苯二酚(C6H6O2)为底物,分析了不同培养料栽培的双孢蘑菇中多酚氧化酶(PPO)的动力学特性。结果表明,蘑菇PPO活力最低的条件为:pH2.0,温度40℃,褐变抑制剂VC浓度0.09%。用均匀设计回归分析程序拟合的不同培养料蘑菇PPO活力与pH值、温度和褐变抑制剂VC浓度间关系的数学方程,置信水平大于95%。Using pyrocatechol as substrate, polyphenoloxidase(PPO) kinetic properties in Agaricus Bisporus cultured by different composts were analysed, the study is to solve the problem of browning in fresh-keeping and processing process.The results showed that when the pH value of reaction system was 2.0,temperature was 40℃ and the concentration of inhibitor(vitamin C) was 0.09%, the activity of polyphenol oxidase in Agaricus bisporus was the lowest. Regression equation was worked out with the program designed by uniform experiment, the confidence level reached to 95%. The equation concerned activity of polyphenol oxidase in Agaricus bisporus cultured by different composts with the change of the pH value of reaction system?temperature and the concentration of inhibitor(vitamin C).

关 键 词：双孢蘑菇 培养料 酶活性抑制 褐变抑制剂 动力学特性 多酚氧化酶 邻苯二酚 分析程序 均匀设计 数学方程 置信水平 PPO pH2 pH值 活力 温度 浓度 VC 

分 类 号：S646.11[农业科学—蔬菜学] S646.14[农业科学—园艺学]