Ca^(2+) cytochemical changes of hepatotoxicity caused by halothane and sevoflurane in enzyme-induced hypoxic rats  

作　　者：Wei-Feng Yu Li-Qun Yang Mai-Tao Zhou Zhi-Qiang Liu Quan Li 

机构地区：[1]Department of Anesthesiology, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200438, China

出　　处：《World Journal of Gastroenterology》2005年第32期5025-5028,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by Military Medical Science Found of China, No.39400126

摘　　要：AIM： To investigat the relation between hepatotoxicity of halothane and sevoflurane and altered hepatic calcium homeostasis in enzyme-induced hypoxic rats. METHODS： Forty-eight rats were pretreated with phenobarbital and randomly divided into six groups （eight in each group） and exposed to O2/N2/1.2 MAC anesthetics for 1 h： normal control （NC）, 21% O2/79% N2; hypoxic control （HC）, 14% O2/86% N2; normal sevoflurane （NS）, 21% O2/ N2/1.2MAC sevoflurane; hypoxic sevoflurane （HS）, 14% O2/N2/1.2MAC sevoflurane; normal halothane （NH）21%O2/79%N2/1.2MAC halothane; hypoxic halothane （HH）, 14%O2/N2/1.2MAC halothane. Liver specimens and blood were taken 24 h after exposure to calcium and determined by EDX microanalysis. RESULTS： The liver of all rats given halothane （14% O2） had extensive centrilobular necrosis and denaturation. Morphologic damage was accompanied with an increase in serum glutarnic pyruvic transminase. In groups NH and HH, more calcium was precipitated in cytoplasm and mitochondria. CONCLUSION： These results suggest that halothane increases cytosolic Ca^2＋ concentration in hepatocytes. Elevation in Ca^2＋ concentration is implicated in the mechanism of halothane-induced hepatotoxicity. sevoflurane is less effective in affecting hepatic calcium homeostasis than halothane.AIM: To investigat the relation between hepatotoxicity of halothane and sevoflurane and altered hepatic calcium homeostasis in enzyme-induced hypoxic rats.METHODS: Forty-eight rats were pretreated with phenobarbital and randomly divided into six groups (eight in each group) and exposed to O2/N2/1.2 MAC anesthetics for :1 h: normal control (NC), 21% O2/79% N2; hypoxic control (HC), 14% O2/86%N2; normal sevoflurane (NS),21% O2/ N2/1.2MAC sevoflurane; hypoxic sevoflurane (HS), 14% O2/N2/1.2MAC sevoflurane; normal halothane (NH)21%O2/7g%N2/1.2MAC halothane; hypoxic halothane (HH), 14% O2/N2/1.2MAC halothane. Liver specimens and blood were taken 24 h after exposure to calcium and determined by EDX microanalysis.RESULTS: The liver of all rats given halothane (14%O2)had extensive centrilobular necrosis and denaturation.Morphologic damage was accompanied with an increase in serum glutamic pyruvic transminase. In groups NH and HH, more calcium was precipitated in cytoplasm and mitochondria.CONCLUSION: These results suggest that halothane increases cytosolic Ca2+ concentration in hepatocytes.Elevation in Ca2+ concentration is implicated in the mechanism of halothane-induced hepatotoxicity.sevoflurane is less effective in affecting hepatic calcium homeostasis than halothane.

关 键 词：Ca^2＋ cytochemistry HEPATOTOXICITY Calcium homeostasis HALOTHANE SEVOFLURANE 

分 类 号：R575[医药卫生—消化系统]