反相高效液相色谱法(RP-HPLC)测定麦汁中的草酸  

Determination of oxalic acid content in wort by reversed-phase HPLC(RP-HPLC)

作　　者：刘景[1] 李崎[1] 顾国贤[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《中国酿造》2005年第10期44-46,共3页China Brewing

摘　　要：利用反相高效液相色谱法(RP-HPLC)测定麦汁中的草酸含量。对各种色谱条件进行了研究,最终确定色谱柱Hypersil ODS 4.0×125mm,流动相为0.5%KH2PO4-0.5mmol/L TBA(tetrabutylammonium salt,四丁基铵盐)水溶液,pH2.0,紫外检测波长220nm。试验证明该方法能有效地分离麦汁中的草酸和酒石酸,准确灵敏、回收率高、重复性好,并且操作简便,可应用于啤酒生产中草酸含量的检测。The oxalic acid in wort was separated and determined by reversed-phase high performance liquid chromatography （RP-HPLC）. A Hypersil ODS 4.0×125 mm column, pH 2.0, an ultraviolet length at 220nm, and a mobile phase containing 0.5% KH2PO4 and 0.5 mmol/L TB （tetrabutylammonium salt） were employed, which allowed a good separation of oxalic acid from tartaric acid in wort. The method was testified to be precise, easily operable and highly reproducible with about 100% recovery, which can be applied to the determination of oxalic acid in beer production.

关 键 词：反相高效液相色谱(RP—HPLC) 草酸 麦汁 

分 类 号：O657.7[理学—分析化学]