酚醛树脂作载体固定化葡萄糖淀粉酶的研究  

THE STUDY ON IMMOBILIZING GLUCOAMYLASE USING PHENOLIC RESIN AS CARRIER

作　　者：王章存[1] 张平之[1] 宋豫军[1] 刘小玲[2] 罗剑超 李道树 

机构地区：[1]郑州轻工业学院食品工程系,郑州450002 [2]郑州轻工业学院食工系

出　　处：《郑州轻工业学院学报》1996年第1期50-54,共5页Journal of Zhengzhou Institute of Light Industry（Natural Science）

摘　　要：以酚醛树脂作载体,研究了糖化型淀粉酶固定化条件及固定化糖化酶的性质.结果表明,在 pH=3,T=35℃,t=4h 条件下所得固定化酶活力最高,与游离酶相比,酶活力回收可达82%.糖化酶经固定后催化反应最适 pH=4.2,最适温度为48℃,K_m=28.6mg/mL,装柱连续水解淀粉240h 酶活力保留87%.The phenolic resin was used as carrier to immobilize the glucoamylase.The factors which effect the process of immobilizing were studied using orthogonal test.The best condi- tion of immobilizing is:pH=3,T=35℃,t=4 h and 82% activity of glucoamylase was con- served.The optimum conditions of immobilized glucoamylase action on the substance (starch)are:pH=4.2,T=48℃.K_■=28.6 mg/mL.Continous hydrolysis amylum 240 h.so 87% activity of glucoamylase was conserved.The result shows the immobilized glucoamylase stability on time is high.

关 键 词：葡萄糖淀粉酶 固相酶 酚醛树脂 载体 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]