反相高效液相色谱法检测酵母胞内V_B_1含量  

Determination of Intracellular Thiamine of Yeast by RP-HPLC

作　　者：肖冬光[1] 毕琳[1] 韩英素[1] 

机构地区：[1]天津科技大学生物工程学院

出　　处：《食品与发酵工业》2005年第10期120-122,共3页Food and Fermentation Industries

摘　　要：采用反相高效液相色谱法测定了酵母胞内VB1的含量。采用ODS2HYPERSIL色谱柱(5μm,250×4.6mm),优化后的色谱条件为:流动相V(甲醇)∶V(水)=3∶7,流速1.2mL/min,波长238nm,柱温35℃。在此条件下回收率为88%～92%。此方法步骤简单,重现性好。A quantitative method was developed for the determination of Vitamin B1 in the yeast cells by RPHPLC. The chromatographic column was ODS-2 HYPERSIL （5 μm,250×4.6 mm） .The optimal chromatographic conditions including methanol and water at 3：7 were served as mobile phase, and flow rate was 1.2 mL/min at 238 nm by using UV-detector; column temperature was 35℃. The methods recovery rate was 88 % 92%. The method is simple and reproducible.

关 键 词：酵母 VB1 反相高效液相色谱法(RP-HPLC) 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]