啤酒酵母β-D-葡聚糖吸附毒素玉米赤霉烯酮(ZEA)的研究  

Adsorptive Properties of β-D-Glucans Extracted from Spent Brewer Yeast Cell Wall against Zearalenone

作　　者：张丽霞[1] 徐学明[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《食品科学》2006年第4期75-78,共4页Food Science

摘　　要：本文用碱法,碱酸法和酶碱法从啤酒废酵母中提取碱不溶性葡聚糖,对产品进行了定性定量分析,得到的产品主要为β-D-葡聚糖,且不含甘露糖。研究了三种产品对真菌毒素玉米赤霉烯酮(ZEA)的吸附作用,实验结果表明,酶碱法提取的葡聚糖具有较好的吸附效果,在反应条件:β-D-葡聚糖100μg/ml,玉米赤霉烯酮40μg/ml,37℃,200r/min振荡2h,吸附量最大可达到2.296μg/mg葡聚糖。β -D-glucans were extracted from the cell wall of spent brewer＇s yeast （Saccharomyces cerevisiae） by alkali, acid-alkali or enzyme-alkali method, respectively. The basic components of the three different extracted products were analyzed. Paper chromatography and IR spectrum analysis showed that D-mannose was not found in all of them. The adsorptive properties of β -D-glucans on Zearalenone（ZEA） were determined by HPLC. It indicated that the absorptive capacity was different to β - D-glucans with different extracting methods. β -D-glucans obtained by enzyme-alkali method showed a relatively high adsorption capacity for ZEA with a maximum adsorption of up to 2.296μ g ZEA/mg glucan.

关 键 词：玉米赤霉烯酮 真菌毒素 啤酒酵母 Β-D-葡聚糖 吸附 

分 类 号：TQ926.1[轻工技术与工程—发酵工程]