褐藻胶裂解酶产生菌的筛选、鉴定及其产酶条件研究  

The Screening, Identification of a Bacillus sp. Strain producing Alginate Lyase and the Research of its Fermentation Conditions

作　　者：张书利[1] 管斌[1] 邱向锋[1] 董书阁[1] 熊三玉[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《现代食品科技》2006年第3期24-27,共4页Modern Food Science and Technology

摘　　要：从土壤中分离得到的一株芽孢杆菌W1在以褐藻酸钠为唯一碳源的培养基中进行驯化后，在琼脂平板上产生透明水解圈，通过筛选得到实验菌株S1，鉴定后确定其为枯草芽孢杆菌。经过发酵培养实验，确定其最佳产酶条件：以蛋白胨和尿素为氮源，褐藻酸钠0．5-0．6％（质量分数），培养初始pH值7.5，培养温度32℃，180r／min摇床发酵84hr-108hr。A Bacillus sp. Stain W1 screened from soil was cultured in the solid medium with the sodium alginate as the sole carbon source. The colony with transparent circle which indicated the production of alginate lyase was chosen as the initial strain S1. The strain S1 was identified as Bacillus subtilis. The research had found that the optimal conditions of producing alginate lyase were as follows. The nitrogen sources were peptone and urea. The optimal content of sodium alginate was 0.5- 0.6%. The alginate lyase was well produced with initial pH 7.5 of liquid medium under 32℃ and rotation rate at 180r/min after fermentation for 84h- 108h.

关 键 词：褐藻胶裂解酶 菌种筛选 菌种鉴定 产酶条件 

分 类 号：TQ925[轻工技术与工程—发酵工程] S986.2[农业科学—捕捞与储运]