A Potential Pathological Role of Angiopoietins Expression in Glomeruli during Progressive Glomerulisclerosis Related to Podocyte Injury  

作　　者：卢远航 邓安国 杨晓 刘建社 朱忠华 

机构地区：[1]Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第3期294-297,共4页华中科技大学学报（医学英德文版）

摘　　要：A potential pathological role of angiopoietins （Ang） in glomeruli following podocyte injury-induced progressive glomerulosclerosis was explored. Eighty male Wistar rats were randomly allocated into sham operation group （Sham, n= 25）, Uninephrectomy group （UPHT, n= 25） and Uninephrectomy＋Daunorubicin group （DRB, n= 30）. In DRB group, daunorubicin （5 mg/kg） was injected via tail vein on the 7th and 14th day after uninephrectomy. At week 1, 2, 4, 6 and 8 respectively following establishment of the animal model, 5 rats in Sham group and UPHT group, and 6 in DRB group were taken respectively for determining 24-h urinary protein excretion rate （24hUPER）, blood urea nitrogen （BUN） and serum creatinine （Scr）. The sections of kidneys were examined by an electric microscope, PAS staining, immunohistochemical staining and in situ hy-bridization histochemistry. The results showed that 24hUPER, BUN and Scr in DRB group were more than those in Sham group and UPHT group at the same time points, and there was a trend towards an increase on level of GSI in DRB group from week 2 to week 8. Electric microscopy revealed that podocyte injury presented in DRB group. The expression of Angl mRNA and protein in glomeruli of DRB group was decreased, while the expression of Ang2 protein in glomeruli of DRB group increased. Meanwhile, the expression of Angl mRNA had a negative correlation with the expression of Ang2 mRNA, and the expression of Angl protein had a positive correlation with the expression of Angl mRNA, and had a negative correlation with 24hUPER, BUN, Scr, glomerular sclerotic index （GSI）, the expression of Ang2 protein and CoIV protein. The expression of Ang2 protein had a positive correlation with the expression of Ang2 mRNA, and had a positive correlation with 24hUPER, BUN, Scr, GSI, the expression of CoIV protein. It was concluded that podocyte injury might lead to an alteration in the expression of Angl and Ang2 within glomeruli. Ang2 may get rid of inhibition from Angl for downregulaA potential pathological role of angiopoietins （Ang） in glomeruli following podocyte injury-induced progressive glomerulosclerosis was explored. Eighty male Wistar rats were randomly allocated into sham operation group （Sham, n= 25）, Uninephrectomy group （UPHT, n= 25） and Uninephrectomy＋Daunorubicin group （DRB, n= 30）. In DRB group, daunorubicin （5 mg/kg） was injected via tail vein on the 7th and 14th day after uninephrectomy. At week 1, 2, 4, 6 and 8 respectively following establishment of the animal model, 5 rats in Sham group and UPHT group, and 6 in DRB group were taken respectively for determining 24-h urinary protein excretion rate （24hUPER）, blood urea nitrogen （BUN） and serum creatinine （Scr）. The sections of kidneys were examined by an electric microscope, PAS staining, immunohistochemical staining and in situ hy-bridization histochemistry. The results showed that 24hUPER, BUN and Scr in DRB group were more than those in Sham group and UPHT group at the same time points, and there was a trend towards an increase on level of GSI in DRB group from week 2 to week 8. Electric microscopy revealed that podocyte injury presented in DRB group. The expression of Angl mRNA and protein in glomeruli of DRB group was decreased, while the expression of Ang2 protein in glomeruli of DRB group increased. Meanwhile, the expression of Angl mRNA had a negative correlation with the expression of Ang2 mRNA, and the expression of Angl protein had a positive correlation with the expression of Angl mRNA, and had a negative correlation with 24hUPER, BUN, Scr, glomerular sclerotic index （GSI）, the expression of Ang2 protein and CoIV protein. The expression of Ang2 protein had a positive correlation with the expression of Ang2 mRNA, and had a positive correlation with 24hUPER, BUN, Scr, GSI, the expression of CoIV protein. It was concluded that podocyte injury might lead to an alteration in the expression of Angl and Ang2 within glomeruli. Ang2 may get rid of inhibition from Angl for downregula

关 键 词：ANGIOPOIETINS GLOMERULOSCLEROSIS DAUNORUBICIN PODOCYTE 

分 类 号：R363[医药卫生—病理学]