根霉淀粉酶的分离纯化及部分性质研究  

Separation and Purification of Rhizopus Amylases and Research of Properties

作　　者：喻凤香[1] 陈煦[1] 林亲录[1] 李珂[1] 杨熠[1] 李菁[1] 

机构地区：[1]湖南农业大学,湖南长沙410128

出　　处：《酿酒》2006年第4期51-53,共3页Liquor Making

摘　　要：本实验对根霉RhizopusRA-1淀粉酶进行了分离纯化,经过(NH4)2SO4分步沉淀,DEAE-纤维素离子交换层析和Sephadex G-100凝胶层析等3部纯化,得到了电泳纯的糖化酶和两条紧密相连的液化型淀粉酶带,用SDS-PAGE法测得三种酶都只含有一种亚基,相对分子质量分别为78000,48000,50000。Amylases of Rhizopus RA-lare separated and purified by （NH4）2SO4 fractional precipitafion,DEAE-cellulose ion-exchange chromatography and gel G-100 filtration.It was determined by PAGE that the amylase shows glucoamylase line and two α -amylase lines, detemined by SDS-PAGE the three molecular weights are about 78kD,48kD and 50kD.

关 键 词：根霉 淀粉酶 提纯 

分 类 号：TS261.12[轻工技术与工程—发酵工程] TS261.15[轻工技术与工程—食品科学与工程]