牛舌菌液体发酵条件的优化研究  

Study on Liquid Fermentative Conditions of Fistulina hepatica

作　　者：陈文强[1] 邓百万[1] 李新生[1] 冯航[2] 

机构地区：[1]陕西理工学院陕西省资源生物重点实验室,陕西汉中723001 [2]西安文理学院生命科学系,陕西西安710065

出　　处：《食品科学》2006年第7期173-176,共4页Food Science

基　　金：陕西省教育厅专项科研基金项目(99JK109)

摘　　要：采用摇瓶培养法对牛舌菌液体发酵的条件进行了研究。通过L9(34)正交试验确定的牛舌菌液体发酵的最适培养基组成为:玉米粉30.0g,黄豆粉7.0g,KH2PO42.0g,MgSO4·7H2O1.0g,VB10.01g;该菌株液体发酵的优化条件为:初始pH4.6～5.4,培养温度28℃,装液量60%,振荡速度210r/min,发酵时间6d,菌丝体生物量达14.7241g/50ml。This paper explore that suitable fermentative conditions, which affect the mycelia growth of Fismlina hepatica. Experimental results indicated that the optimum composition of a fermentative medium is as following： corn powder 30.0g, soya powder 7.0g, KH2PO4 2.0g, MgSO4 · 7H2O 1.0g, VB1 0.01g; pH4.6～5.4, the shaker rotation speed is at 210r/min, the temperature is at 28℃, the optimal culture media capacity is 60%. The highest yield of mycelia was obtained after fermentation for 6 days, the weight of mycelia reached 14.7241g/50ml fermentation liquid.

关 键 词：牛舌菌 液体发酵 菌丝体 

分 类 号：TS201.3[轻工技术与工程—食品科学]