豆豉纤溶酶生产工艺研究  

Preparation of Fibrinolytic Enzyme from Fermented Soybean

作　　者：孟凡涛[1] 陶琳[1] 吴思方[1] 赵学文[1] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430064

出　　处：《中国医药工业杂志》2006年第8期525-527,共3页Chinese Journal of Pharmaceuticals

摘　　要：以筛选得的纤溶酶产生菌——枯草芽孢杆菌HGD107进行15L发酵罐生产豆豉纤溶酶,产物经离心、D392型树脂脱色、盐析、超滤等操作纯化,制得冻干豆豉纤溶酶产品,其比活力为530．6u/mg,纯化倍数11,活性回收率65．7%。A strain designed as Bacillus subtilis HGD107 was fermented in 15L fermenter to prepare the fibrinolytic enzyme. The enzyme was purified by centrifugation, decoloration with ion exchange resin D392, salting out with ammonium sulfate and ultrafiltration, and finally lyophilized. The specific activity was 530.6u/mg with 65.7 % activity recovery and 11 fold of purification.

关 键 词：纤溶酶 豆豉 发酵 

分 类 号：R977.3[医药卫生—药品]